Anticorps Monoclonal anti-BAX

• Phare
• Validé par KD/KO

BAX Monoclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, rat, souris et plus (4)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications(558)

CloneNo.

4G5E8

N° de cat : 60267-1-Ig

Synonymes

4G5E8, Apoptosis regulator BAX, Bcl2-L-4, Bcl-2-like protein 4

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Galerie de données de validation

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  WB analysis using 60267-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60267-1-Ig (BAX antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis using 60267-1-Ig

  EC109 cells were subjected to SDS PAGE followed by western blot with 60267-1-Ig (BAX antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 60267-1-Ig

  WB result of BAX antibody (60267-1-Ig; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-BAX transfected HeLa cells.

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  WB analysis of human testis using 60267-1-Ig

  human testis tissue were subjected to SDS PAGE followed by western blot with 60267-1-Ig (BAX antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 60267-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 60267-1-Ig (BAX antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of THP-1 using 60267-1-Ig

  IP result of anti-BAX (IP:60267-1-Ig, 4ug; Detection:60267-1-Ig 1:6000) with THP-1 cells lysate 1520 ug.

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  IHC staining of human liver cancer using 60267-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60267-1-Ig (BAX antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 60267-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60267-1-Ig (BAX antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 60267-1-Ig

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 60267-1-Ig (BAX antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 60267-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60267-1-Ig (BAX antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 60267-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 60267-1-Ig (BAX antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 60267-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60267-1-Ig (BAX antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human rectal cancer using 60267-1-Ig

  Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 60267-1-Ig (BAX antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of MCF-7 using 60267-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using BAX antibody (60267-1-Ig, Clone: 4G5E8 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules COLO 320, cellules EC109, cellules HEK-293, cellules HepG2, cellules Neuro-2a, cellules PC-12, cellules ROS1728, tissu testiculaire humain
• Résultats positifs en IP: cellules THP-1,
• Résultats positifs en IHC: tissu de cancer du foie humain, tissu de cancer de l'estomac humain, tissu de cancer du côlon humain, tissu de cancer du poumon humain, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules MCF-7,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:20000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 531 publications below
• IHC: See 29 publications below
• CoIP: See 1 publications below

Informations sur le produit

60267-1-Ig cible BAX dans les applications de WB, IHC, IF/ICC, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, canin, Humain, Lapin, porc, souris, Hamster
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: BAX Protéine recombinante Ag21068
• Nom complet: BCL2-associated X protein
• Masse moléculaire calculée: 21 kDa
• Poids moléculaire observé: 21 kDa
• Numéro d’acquisition GenBank: BC014175
• Symbole du gène: BAX
• Identification du gène (NCBI): 581
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

BAX, also named as BCL2L4, is a pro-apoptotic member of the Bcl-2 protein family, which plays a pivotal role in controlling cell life and death. Bax largely localizes to the cytoplasm of healthy cells, but accumulates on the outer mitochondrial membrane upon apoptosis induction (PMID: 9108035). BAX can commit a cell to apoptosis by translocation from the cytosol to the mitochondria and permeabilization of the outer mitochondrial membrane, which leads to the release of cytochrome c from mitochondria (PMID: 21763611). The expression of BAX is upregulated by the tumor suppressor protein p53, and BAX has been shown to be involved in p53-mediated apoptosis (PMID: 8183579).

Publications

Species	Application	Title
human	WB	Adv Sci (Weinh); Mitochondrial tRNAGlu 14693A>G Mutation, an "Enhancer" to the Phenotypic Expression of Leber's Hereditary Optic Neuropathy; Authors - Lihao Jin
rat,mouse	WB,IHC	Acta Pharm Sin B; Protocatechuic aldehyde protects cardiomycoytes against ischemic injury via regulation of nuclear pyruvate kinase M2.; Authors - Xunxun Wu
human	WB	Cell Death Differ; ZNF451 collaborates with RNF8 to regulate RNF168 localization and amplify ubiquitination signaling to promote DNA damage repair and regulate radiosensitivity; Authors - Feng Xu
rat	WB	J Adv Res; Evolocumab prevents atrial fibrillation in rheumatoid arthritis rats through restraint of PCSK9 induced atrial remodeling; Authors - Xuejie Han
	WB	J Nanobiotechnology; Nanocomposites based on nanoceria regulate the immune microenvironment for the treatment of polycystic ovary syndrome; Authors - Sisi Yan
human	WB	Redox Biol; Mecheliolide elicits ROS-mediated ERS driven immunogenic cell death in hepatocellular carcinoma.; Authors - Zhongren Xu

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