Anticorps Recombinant de lapin anti-BAZ2A

BAZ2A Recombinant Antibody for IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

230205H8

N° de cat : 82910-1-RR

Synonymes

WALp3, TTF I interacting protein 5, TIP5, KIAA0314, hWALp3

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Galerie de données de validation

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  IF Staining of HepG2 using 82910-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using BAZ2A antibody (82910-1-RR, Clone: 230205H8 ) at dilution of 1:500 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM004).(CL488-Phalloidin, green).

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  IF Staining of A549 using 82910-1-RR

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using BAZ2A antibody (82910-1-RR, Clone: 230205H8 ) at dilution of 1:500 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM004).(CL488-Phalloidin, green).

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  Affinity and Kinetic Characterization of 82910-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82910-1-RR against Human BAZ2A were performed. The affinity constant is 1.46 nM.

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Applications testées

• Résultats positifs en IF/ICC: cellules HepG2, cellules A549

Dilution recommandée

• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

82910-1-RR cible BAZ2A dans les applications de IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: BAZ2A Protéine recombinante Ag33963
• Nom complet: bromodomain adjacent to zinc finger domain, 2A
• Masse moléculaire calculée: 211 kDa
• Numéro d’acquisition GenBank: NM_013449.4
• Symbole du gène: BAZ2A
• Identification du gène (NCBI): 11176
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

BAZ2A (bromodomain adjacent to zinc finger domain 2A), also known as TIP5. It is expected to be located in nucleus, and the protein is expressed at moderate levels in most tissues analyzed, including heart, brain, placenta, lung, skeletal muscle, kidney and pancreas. BAZ2A is an epigenetic regulator affecting transcription of ribosomal RNA, it is also a regulatory subunit of the ATP-dependent NoRC-1 and NoRC-5 ISWI chromatin remodeling complexes, which form ordered nucleosome arrays on chromatin and facilitate access to DNA during DNA-templated processes such as DNA replication, transcription, and repair (PubMed:28801535). The calculated molecular weight of BAZ2A is 210 kDa.

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