Anticorps Recombinant de lapin anti-BAZ2A

BAZ2A Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

230205B8

N° de cat : 82910-2-RR

Synonymes

230205B8, hWALp3, KIAA0314, TIP5, TTF I interacting protein 5

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Galerie de données de validation

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  WB analysis using 82910-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82910-2-RR (BAZ2A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of A549 using 82910-2-RR

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using BAZ2A antibody (82910-2-RR, Clone: 230205B8 ) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  IF Staining of U2OS using 82910-2-RR

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using BAZ2A antibody (82910-2-RR, Clone: 230205B8 ) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  FC experiment of U-2 OS using 82910-2-RR

  (blue)1x10^6 U-2 OS were intracellularly stained with 0.25 ug Anti-Human BAZ2A (82910-2-RR, Clone:230205B8) and Alexa Fluor™ Plus 647-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:166(red), or 0.25 ug rabbit IgG isotype control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 82910-2-RR

  Biolayer interferometry (BLl) kinetic assays of 82910-2-RR against Human BAZ2A were performed. The affinity constant is 1.46 nM.

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Applications testées

• Résultats positifs en WB: cellules HEK-293T, cellules HeLa, cellules K-562, cellules MDA-MB-231, cellules PC-3
• Résultats positifs en IF/ICC: cellules U2OS, cellules A549
• Résultats positifs en FC (Intra): U-2 OS,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

82910-2-RR cible BAZ2A dans les applications de WB, IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: BAZ2A Protéine recombinante Ag33963
• Nom complet: bromodomain adjacent to zinc finger domain, 2A
• Masse moléculaire calculée: 211 kDa
• Poids moléculaire observé: 250-270 kDa
• Numéro d’acquisition GenBank: NM_013449.4
• Symbole du gène: BAZ2A
• Identification du gène (NCBI): 11176
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

BAZ2A (bromodomain adjacent to zinc finger domain 2A), also known as TIP5. It is expected to be located in nucleus, and the protein is expressed at moderate levels in most tissues analyzed, including heart, brain, placenta, lung, skeletal muscle, kidney and pancreas. BAZ2A is an epigenetic regulator affecting transcription of ribosomal RNA, it is also a regulatory subunit of the ATP-dependent NoRC-1 and NoRC-5 ISWI chromatin remodeling complexes, which form ordered nucleosome arrays on chromatin and facilitate access to DNA during DNA-templated processes such as DNA replication, transcription, and repair (PubMed:28801535). The calculated molecular weight of BAZ2A is 210 kDa.

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