Anticorps Polyclonal de lapin anti-BBOF1

BBOF1 Polyclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 24337-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of TT using 24337-1-AP (same clone as 24337-1-PBS)

  TT cells were subjected to SDS PAGE followed by western blot with 24337-1-AP (CCDC176 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24337-1-PBS in a different storage buffer formulation.

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  WB analysis using 24337-1-AP (same clone as 24337-1-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 24337-1-AP (BBOF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24337-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 24337-1-AP (same clone as 24337-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 24337-1-AP (BBOF1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24337-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 24337-1-AP (same clone as 24337-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 24337-1-AP (BBOF1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24337-1-PBS in a different storage buffer formulation.

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Informations sur le produit

24337-1-PBS cible BBOF1 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: BBOF1 Protéine recombinante Ag19302
• Nom complet: chromosome 14 open reading frame 45
• Masse moléculaire calculée: 529 aa, 62 kDa
• Poids moléculaire observé: 62 kDa
• Numéro d’acquisition GenBank: BC104981
• Symbole du gène: BBOF1
• Identification du gène (NCBI): 80127
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Basal body orientation factor 1 (BBOF1), also known as CCDC176, contains two coiled-coil domains and is evolutionarily conserved in vertebrates. Recently, Xenopus bbof1 has been characterized as a cilia-associated factor that is induced by the transcription factor foxj1 to mediate the alignment and orientation of cilia in epidermal multi-ciliated cells. BBOF1 is required for sperm flagellar axoneme maintenance and male fertility in mice. BBOF1 expression is elevated during meiosis, and it is accumulated in round spermatids. BBOF1 deletion has compromised effects on growth, survival, and spermatogenesis.

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