Anticorps Monoclonal anti-BBS10

BBS10 Monoclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

4C6A10

N° de cat : 66679-1-PBS

Synonymes

Bardet Biedl syndrome 10, BBS10, C12orf58, FLJ23560

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Galerie de données de validation

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  WB analysis using 66679-1-Ig (same clone as 66679-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66679-1-Ig (BBS10 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 66679-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66679-1-Ig (same clone as 66679-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66679-1-Ig (BBS10 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66679-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 66679-1-Ig (same clone as 66679-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66679-1-Ig (BBS10 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66679-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66679-1-Ig (same clone as 66679-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66679-1-Ig (BBS10 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66679-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66679-1-PBS cible BBS10 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: BBS10 Protéine recombinante Ag17960
• Nom complet: Bardet-Biedl syndrome 10
• Masse moléculaire calculée: 81 kDa
• Poids moléculaire observé: 81 kDa
• Numéro d’acquisition GenBank: BC013795
• Symbole du gène: BBS10
• Identification du gène (NCBI): 79738
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

BBS10, also named as C12orf58, belongs to the TCP-1 chaperonin family. It is probable molecular chaperone. Assists the folding of proteins upon ATP hydrolysis. As part of the BBS/CCT complex, BBS10 may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. Involved in adipogenic differentiation. (PMID: 20080638, 19190184) Defects in BBS10 are the cause of Bardet-Biedl syndrome type 10 (BBS10).

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