Anticorps Polyclonal de lapin anti-MKS1

MKS1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

26

N° de cat : 16206-1-AP

Synonymes

BBS13, Meckel syndrome type 1 protein, Meckel syndrome, type 1, MES, MKS, MKS1

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Galerie de données de validation

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  WB analysis of mouse brain using 16206-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 16206-1-AP (MKS1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse uterus using 16206-1-AP

  mouse uterus tissue were subjected to SDS PAGE followed by western blot with 16206-1-AP (MKS1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 16206-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 16206-1-AP (MKS1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 16206-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16206-1-AP (MKS1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 16206-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 16206-1-AP (MKS1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 16206-1-AP

  IP Result of anti-MKS1 (IP:16206-1-AP, 3ug; Detection:16206-1-AP 1:1000) with HEK-293 cells lysate 4500ug.

• 

  IHC staining of human liver cancer using 16206-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16206-1-AP (MKS1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 16206-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16206-1-AP (MKS1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 16206-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 16206-1-AP (MKS1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver cancer using 16206-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 16206-1-AP (MKS1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of hTERT-RPE1 cells and Mouse embryonic fibroblasts using 16206-1-AP

  IF result from Dr. Corbit, Kevin. anti-MKS1 (16206-1-AP) marks the transition zone of Human hTERT-RPE1 cells and Mouse embryonic fibroblasts.

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, cellules HEK-293, cellules HeLa, cellules SH-SY5Y, tissu utérin de souris
• Résultats positifs en IP: cellules HEK-293,
• Résultats positifs en IHC: tissu de cancer du foie humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules hTERT-RPE1 et fibroblastes embryonnaires de souris

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 7 publications below
• IF: See 21 publications below

Informations sur le produit

16206-1-AP cible MKS1 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MKS1 Protéine recombinante Ag9177
• Nom complet: Meckel syndrome, type 1
• Masse moléculaire calculée: 559 aa, 65 kDa
• Poids moléculaire observé: 65-70 kDa
• Numéro d’acquisition GenBank: BC010061
• Symbole du gène: MKS1
• Identification du gène (NCBI): 54903
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MKS1 (Meckel syndrome type 1 protein) is a 559-amino acid protein that contains a conserved B9 domain. It is a component of a large protein complex which localizes to the ciliary transition zone and regulates mammalian ciliogenesis and ciliary membrane composition (PMID: 21725307). MKS1 is required for ciliary structure and function, and is involved in centrosome migration to the apical cell surface during early ciliogenesis (PMID: 17185389; 19515853). Broad tissue expression of the MKS1 gene has been reported (PMID: 16415886). Defects in MKS1 are the cause of Meckel syndrome type 1 (MKS1), an autosomal recessive lethal malformation syndrome characterized by renal cystic dysplasia, central nervous system malformations, and hepatic developmental defects (PMID: 16415886). In addition, defects in MKS1 are also the cause of Bardet-Biedl syndrome type 13 (BBS13) (PMID: 18327255).

Publications

Species	Application	Title
human,mouse	WB,IF	Nat Genet; A transition zone complex regulates mammalian ciliogenesis and ciliary membrane composition.; Authors - Garcia-Gonzalo Francesc R FR
mouse	IF	Cell; The spinocerebellar ataxia-associated gene Tau tubulin kinase 2 controls the initiation of ciliogenesis.; Authors - Goetz Sarah C SC
mouse	IF	Nat Cell Biol; Lineage specificity of primary cilia in the mouse embryo.; Authors - Fiona K Bangs
human	IF	Sci Adv; Ciliopathy protein HYLS1 coordinates the biogenesis and signaling of primary cilia by activating the ciliary lipid kinase PIPKIγ.; Authors - Chuan Chen
human	IF	Nat Commun; Microtubule asters anchored by FSD1 control axoneme assembly and ciliogenesis.; Authors - Hai-Qing Tu
human	IF	Nat Commun; Phosphatidylinositol phosphate kinase PIPKIγ and phosphatase INPP5E coordinate initiation of ciliogenesis.; Authors - Qingwen Xu