Anticorps Polyclonal de lapin anti-BBS2

BBS2 Polyclonal Antibody for IF, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

12

Avis (1)

N° de cat : 11188-2-AP

Synonymes

Bardet Biedl syndrome 2, BBS, BBS2

---

Galerie de données de validation

• 

  WB analysis using 11188-2-AP

  Mouse testis lysates were subjected to SDS PAGE followed by western blot with 11188-2-AP (BBS2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse testis using 11188-2-AP

  IP Result of anti-BBS2 (IP:11188-2-AP, 4ug; Detection:11188-2-AP 1:300) with mouse testis tissue lysate 4000ug.

• 

  IF Staining of hTERT-RPE1 cells using 11188-2-AP

  IF result (cilia stain) of anti-BBS2 (11188-2-AP, 1:50) with serum-starved hTERT-RPE1 cells by Dr. Moshe Kim.

---

Applications testées

• Résultats positifs en WB: tissu testiculaire de souris,
• Résultats positifs en IP: tissu testiculaire de souris
• Résultats positifs en IF: cellules hTERT-RPE1

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IHC: See 2 publications below
• IF: See 8 publications below

Informations sur le produit

11188-2-AP cible BBS2 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: BBS2 Protéine recombinante Ag1662
• Nom complet: Bardet-Biedl syndrome 2
• Masse moléculaire calculée: 80 kDa
• Poids moléculaire observé: 80 kDa
• Numéro d’acquisition GenBank: BC014140
• Symbole du gène: BBS2
• Identification du gène (NCBI): 583
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

BBS2 is one of seven BBS proteins that form the stable core of a protein complex (BBSome complex, 438kd) which is required for ciliogenesis but is dispensable for centriolar satellite function.

Publications

Species	Application	Title
human	IF	Cell; The conserved Bardet-Biedl syndrome proteins assemble a coat that traffics membrane proteins to cilia.; Authors - Jin Hua H
mouse	IF	Cell Stem Cell; Asymmetric segregation of the double-stranded RNA binding protein Staufen2 during mammalian neural stem cell divisions promotes lineage progression.; Authors - Kusek Gretchen G
mouse	IF	J Cell Biol; Ubiquitin links smoothened to intraflagellar transport to regulate Hedgehog signaling.; Authors - Paurav B Desai
mouse	IHC	Brain Struct Funct; A novel form of ciliopathy underlies hyperphagia and obesity in Ankrd26 knockout mice.; Authors - Peter Acs
mouse	IF	Hum Mol Genet; Essential role of nephrocystin in photoreceptor intraflagellar transport in mouse.; Authors - Jiang Si-Tse ST
mouse	IF	FASEB J; Genetic interaction of mammalian IFT-A paralogs regulates cilia disassembly, ciliary entry of membrane protein, Hedgehog signaling, and embryogenesis.; Authors - Wei Wang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

Deng (Verified Customer) (07-30-2019)

This antibody stained primary cilia in cultured IMCD3 cells.

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1;250
• Cell Tissue Type: IMCD3 cells

BBS2 Antibody Immunofluorescence, validation (1;250 dilution) in IMCD3 cells (Cat no:11188-2-AP)