Anticorps Polyclonal de lapin anti-BBS3

• Phare
• Validé par KD/KO

BBS3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

canin, Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 12676-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of rat brain using 12676-1-AP (same clone as 12676-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 12676-1-AP (BBS3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12676-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 12676-1-AP (same clone as 12676-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12676-1-AP (BBS3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12676-1-PBS in a different storage buffer formulation.

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  WB analysis of human brain using 12676-1-AP (same clone as 12676-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 12676-1-AP (BBS3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12676-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293T using 12676-1-AP (same clone as 12676-1-PBS)

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 12676-1-AP (BBS3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12676-1-PBS in a different storage buffer formulation.

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  IP experiment of rat brain using 12676-1-AP (same clone as 12676-1-PBS)

  IP result of anti-BBS3 (IP:12676-1-AP, 3ug; Detection:12676-1-AP 1:600) with rat brain tissue lysate 5200ug. This data was developed using the same antibody clone with 12676-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 12676-1-AP (same clone as 12676-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12676-1-AP (BBS3 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 12676-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 12676-1-AP (same clone as 12676-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12676-1-AP (BBS3 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 12676-1-PBS in a different storage buffer formulation.

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  IF Staining of hTERT-RPE1 cells using 12676-1-AP (same clone as 12676-1-PBS)

  IF result (cytoplasm and the base of cilia stain) of anti-BBS3 (12676-1-AP; 1:50) with hTERT-RPE1 cell (MeOH fixed) by Dr. Moshe Kim. This data was developed using the same antibody clone with 12676-1-PBS in a different storage buffer formulation.

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  IF Staining of MDCK using 12676-1-AP (same clone as 12676-1-PBS)

  Immunofluorescent analysis of MDCK cells using 12676-1-AP (BBS3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12676-1-PBS in a different storage buffer formulation.

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Informations sur le produit

12676-1-PBS cible BBS3 dans les applications de WB, IHC, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons canin, Humain, rat, souris

• Réactivité: canin, Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: BBS3 Protéine recombinante Ag3363
• Nom complet: ADP-ribosylation factor-like 6
• Masse moléculaire calculée: 186 aa, 21 kDa
• Poids moléculaire observé: 21 kDa
• Numéro d’acquisition GenBank: BC024239
• Symbole du gène: ARL6/BBS3
• Identification du gène (NCBI): 84100
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

ADP-ribosylation factor-like protein 6 (ARL6), belongs to a small GTPase superfamily, is involved in membrane protein trafficking at the base of the ciliary organelle. ARL6 is also named Bardet-Biedl syndrome 3 protein, because defects in ARL6 are a cause of Bardet-Biedl syndrome type 3 (BBS3), which is a genetically heterogeneous disorder characterized by usually severe pigmentary retinopathy, early onset obesity, polydactyly, hypogenitalism, renal malformation and mental retardation. ARL6 can interact with many ARL6 interacting proteins and form BBSome complex with other BBS proteins including BBS1, BBS2, BBS4 and so on.

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