Anticorps Polyclonal de lapin anti-BBS4

• Phare
• Validé par KD/KO

BBS4 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 12766-1-PBS

Synonymes

Bardet Biedl syndrome 4, Bardet-Biedl syndrome 4 protein

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Galerie de données de validation

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  WB analysis using 12766-1-AP (same clone as 12766-1-PBS)

  HEK-293 cell lysates were subjected to SDS PAGE followed by western blot with 12766-1-AP (BBS4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12766-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse kidney using 12766-1-AP (same clone as 12766-1-PBS)

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12766-1-AP (BBS4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12766-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse stomach using 12766-1-AP (same clone as 12766-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 12766-1-AP (BBS4 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12766-1-PBS in a different storage buffer formulation.

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  IHC staining of rat kidney using 12766-1-AP (same clone as 12766-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 12766-1-AP (BBS4 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12766-1-PBS in a different storage buffer formulation.

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  IF Staining of hTERT-RPE1 cells using 12766-1-AP (same clone as 12766-1-PBS)

  IF result (cytoplasm and the length of cilia cilia stain) of anti-BBS4 (12766-1-AP; 1:50) with serum-starved hTERT-RPE1 cell (MeOH fixed) by Dr. Moshe Kim. This data was developed using the same antibody clone with 12766-1-PBS in a different storage buffer formulation.

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  IF Staining of hTERT-RPE1 using 12766-1-AP (same clone as 12766-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using BBS4 antibody (12766-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CoraLite®594 ARL13B antibody (CL594-17711, red). This data was developed using the same antibody clone with 12766-1-PBS in a different storage buffer formulation.

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Informations sur le produit

12766-1-PBS cible BBS4 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: BBS4 Protéine recombinante Ag3430
• Nom complet: Bardet-Biedl syndrome 4
• Masse moléculaire calculée: 519 aa, 58 kDa
• Poids moléculaire observé: 50-60 kDa
• Numéro d’acquisition GenBank: BC027624
• Symbole du gène: BBS4
• Identification du gène (NCBI): 585
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

BBS4 (Bardet-Biedl syndrome protein 4) is one of the early BBS proteins discovered, with 35 mutations reported to cause the BBS phenotype (PMID: 26762677). BBS4 undergoes localization to the centriolar satellites of centrosomes and basal body of primary cilia, where it takes part in recruiting cargo to centriolar satellites and allowing the formation of a functional centrosomal microtubule organizing center (MTOC) (PMID: 25860617). Some research has found that BBS4 expression in humans during the development of CNS and sensory organs, concerning the cardinal clinical signs of BBS (PMID: 33860840).

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