Anticorps Polyclonal de lapin anti-BBS5

• Phare
• Validé par KD/KO

BBS5 Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugaison

Non conjugué

Publications

26

N° de cat : 14569-1-AP

Synonymes

Bardet Biedl syndrome 5, BBS5

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Galerie de données de validation

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  WB analysis of mouse retina using 14569-1-AP

  mouse retina tissue were subjected to SDS PAGE followed by western blot with 14569-1-AP (BBS5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat testis using 14569-1-AP

  rat testis tissue were subjected to SDS PAGE followed by western blot with 14569-1-AP (BBS5 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 14569-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 14569-1-AP (BBS5 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 14569-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 14569-1-AP (BBS5 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse eye using 14569-1-AP

  mouse eye tissue were subjected to SDS PAGE followed by western blot with 14569-1-AP (BBS5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse testis using 14569-1-AP

  IP result of anti-BBS5(IP:14569-1-AP, 4ug; Detection:14569-1-AP 1:1500) with mouse testis tissue lysate 1120 ug.

• 

  IHC staining of human testis using 14569-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 14569-1-AP (BBS5 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human testis using 14569-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 14569-1-AP (BBS5 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of mouse kidney using 14569-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 14569-1-AP (BBS5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 14569-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 14569-1-AP (BBS5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 14569-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14569-1-AP (BBS5 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human kidney using 14569-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14569-1-AP (BBS5 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human stomach using 14569-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 14569-1-AP (BBS5 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human stomach using 14569-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 14569-1-AP (BBS5 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of mouse testis using 14569-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14569-1-AP (BBS5 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 14569-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14569-1-AP (BBS5 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu rétinien de souris, tissu oculaire de souris, tissu testiculaire de rat, tissu testiculaire de souris, tissu testiculaire humain
• Résultats positifs en IP: tissu testiculaire de souris,
• Résultats positifs en IHC: tissu testiculaire humain, tissu d'estomac humain, tissu rénal de souris, tissu rénal humain, tissu testiculaire de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 13 publications below
• IHC: See 1 publications below
• IF: See 18 publications below
• CoIP: See 1 publications below

Informations sur le produit

14569-1-AP cible BBS5 dans les applications de WB, IP, IHC, IF, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: BBS5 Protéine recombinante Ag6153
• Nom complet: Bardet-Biedl syndrome 5
• Masse moléculaire calculée: 39 kDa
• Poids moléculaire observé: 39 kDa
• Numéro d’acquisition GenBank: BC044593
• Symbole du gène: BBS5
• Identification du gène (NCBI): 129880
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

BBS5 encodes a protein that has been directly linked to Bardet-Biedl syndrome. Bardet-Biedl syndrome (BBS) is an autosomal recessive condition characterised by rod-cone dystrophy, postaxial polydactyly, central obesity, mental retardation, hypogonadism, and renal dysfunction. Other associated clinical findings in BBS patients include diabetes, hypertension and congenital heart defects. BBS expression varies both within and between families and diagnosis is often difficult. Experimentation in non-human eukaryotes suggests that BBS5 is expressed in ciliated cells and that it is required for the formation of cilia. Alternate transcriptional splice variants have been observed but have not been fully characterized.

Publications

Species	Application	Title
human	IF	Nat Genet; A transition zone complex regulates mammalian ciliogenesis and ciliary membrane composition.; Authors - Garcia-Gonzalo Francesc R FR
human	IF	Cell; The conserved Bardet-Biedl syndrome proteins assemble a coat that traffics membrane proteins to cilia.; Authors - Jin Hua H
human	WB	Nat Cell Biol; Systematic proteomics of the VCP-UBXD adaptor network identifies a role for UBXN10 in regulating ciliogenesis.; Authors - Malavika Raman
mouse	WB,IF	Dev Cell; IFT27 Links the BBSome to IFT for Maintenance of the Ciliary Signaling Compartment.; Authors - Thibaut Eguether
mouse	IF	Dev Cell; The Intraflagellar Transport Protein IFT27 Promotes BBSome Exit from Cilia through the GTPase ARL6/BBS3.; Authors - Gerald M Liew
rat	IF	Nat Struct Mol Biol; Structural basis for membrane targeting of the BBSome by ARL6.; Authors - André Mourão

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