Anticorps Polyclonal de lapin anti-BBS5

• Phare
• Validé par KD/KO

BBS5 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 14569-1-PBS

Synonymes

Bardet-Biedl syndrome 5 protein, Bardet Biedl syndrome 5

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Galerie de données de validation

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  WB analysis of mouse retina using 14569-1-AP (same clone as 14569-1-PBS)

  mouse retina tissue were subjected to SDS PAGE followed by western blot with 14569-1-AP (BBS5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14569-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse eye using 14569-1-AP (same clone as 14569-1-PBS)

  mouse eye tissue were subjected to SDS PAGE followed by western blot with 14569-1-AP (BBS5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14569-1-PBS in a different storage buffer formulation.

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  WB analysis of human testis using 14569-1-AP (same clone as 14569-1-PBS)

  human testis tissue were subjected to SDS PAGE followed by western blot with 14569-1-AP (BBS5 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14569-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse testis using 14569-1-AP (same clone as 14569-1-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 14569-1-AP (BBS5 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14569-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat testis using 14569-1-AP (same clone as 14569-1-PBS)

  rat testis tissue were subjected to SDS PAGE followed by western blot with 14569-1-AP (BBS5 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14569-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse testis using 14569-1-AP (same clone as 14569-1-PBS)

  IP result of anti-BBS5 (IP:14569-1-AP, 4ug; Detection:14569-1-AP 1:1500) with mouse testis tissue lysate 1120 ug. This data was developed using the same antibody clone with 14569-1-PBS in a different storage buffer formulation.

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  IHC staining of human testis using 14569-1-AP (same clone as 14569-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 14569-1-AP (BBS5 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 14569-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human testis using 14569-1-AP (same clone as 14569-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 14569-1-AP (BBS5 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 14569-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach using 14569-1-AP (same clone as 14569-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 14569-1-AP (BBS5 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14569-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach using 14569-1-AP (same clone as 14569-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 14569-1-AP (BBS5 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14569-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 14569-1-AP (same clone as 14569-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 14569-1-AP (BBS5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14569-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 14569-1-AP (same clone as 14569-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 14569-1-AP (BBS5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14569-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 14569-1-AP (same clone as 14569-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14569-1-AP (BBS5 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14569-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 14569-1-AP (same clone as 14569-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14569-1-AP (BBS5 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14569-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 14569-1-AP (same clone as 14569-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14569-1-AP (BBS5 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 14569-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 14569-1-AP (same clone as 14569-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14569-1-AP (BBS5 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 14569-1-PBS in a different storage buffer formulation.

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Informations sur le produit

14569-1-PBS cible BBS5 dans les applications de WB, IHC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: BBS5 Protéine recombinante Ag6153
• Nom complet: Bardet-Biedl syndrome 5
• Masse moléculaire calculée: 39 kDa
• Poids moléculaire observé: 39 kDa
• Numéro d’acquisition GenBank: BC044593
• Symbole du gène: BBS5
• Identification du gène (NCBI): 129880
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

BBS5 encodes a protein that has been directly linked to Bardet-Biedl syndrome. Bardet-Biedl syndrome (BBS) is an autosomal recessive condition characterised by rod-cone dystrophy, postaxial polydactyly, central obesity, mental retardation, hypogonadism, and renal dysfunction. Other associated clinical findings in BBS patients include diabetes, hypertension and congenital heart defects. BBS expression varies both within and between families and diagnosis is often difficult. Experimentation in non-human eukaryotes suggests that BBS5 is expressed in ciliated cells and that it is required for the formation of cilia. Alternate transcriptional splice variants have been observed but have not been fully characterized.

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