Anticorps Recombinant de lapin anti-BBS5

BBS5 Recombinant Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

240674B5

N° de cat : 83722-1-RR

Synonymes

240674B5, Bardet Biedl syndrome 5, Bardet-Biedl syndrome 5 protein

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Galerie de données de validation

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  WB analysis using 83722-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83722-1-RR (BBS5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 83722-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83722-1-RR (BBS5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IF Staining of MDCK using 83722-1-RR

  Immunofluorescent analysis of (4% PFA) fixed MDCK cells using BBS5 antibody (83722-1-RR, Clone: 240674B5 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CoraLite®594 ARL13B antibody (CL594-17711, red).

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Applications testées

• Résultats positifs en WB: tissu rétinien de souris, tissu oculaire de souris, tissu rénal de souris, tissu testiculaire de rat, tissu testiculaire de souris
• Résultats positifs en IF/ICC: cellules MDCK,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

83722-1-RR cible BBS5 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: BBS5 Protéine recombinante Ag6153
• Nom complet: Bardet-Biedl syndrome 5
• Masse moléculaire calculée: 39 kDa
• Poids moléculaire observé: 39 kDa
• Numéro d’acquisition GenBank: BC044593
• Symbole du gène: BBS5
• Identification du gène (NCBI): 129880
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

BBS5 encodes a protein that has been directly linked to Bardet-Biedl syndrome. Bardet-Biedl syndrome (BBS) is an autosomal recessive condition characterised by rod-cone dystrophy, postaxial polydactyly, central obesity, mental retardation, hypogonadism, and renal dysfunction. Other associated clinical findings in BBS patients include diabetes, hypertension and congenital heart defects. BBS expression varies both within and between families and diagnosis is often difficult. Experimentation in non-human eukaryotes suggests that BBS5 is expressed in ciliated cells and that it is required for the formation of cilia. Alternate transcriptional splice variants have been observed but have not been fully characterized.

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