Anticorps Polyclonal de lapin anti-BBX

• Phare
• Validé par KD/KO

BBX Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (2)

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 17254-1-AP

Synonymes

BBX, Bobby sox homolog, bobby sox homolog (Drosophila), HBP2, HMG box containing protein 2, HSPC339, MDS001

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Galerie de données de validation

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  WB analysis of HepG2 using 17254-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 17254-1-AP (BBX antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 17254-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 17254-1-AP (BBX antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 17254-1-AP

  IP Result of anti-BBX (IP:17254-1-AP, 4ug; Detection:17254-1-AP 1:1000) with HepG2 cells lysate 2400ug.

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  IHC staining of human ovary using 17254-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 17254-1-AP (BBX antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human ovary using 17254-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 17254-1-AP (BBX antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human placenta using 17254-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 17254-1-AP (BBX antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human placenta using 17254-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 17254-1-AP (BBX antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human brain using 17254-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 17254-1-AP (BBX antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human brain using 17254-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 17254-1-AP (BBX antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human spleen using 17254-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 17254-1-AP (BBX antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human spleen using 17254-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 17254-1-AP (BBX antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human lung using 17254-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 17254-1-AP (BBX antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human lung using 17254-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 17254-1-AP (BBX antibody) at dilution of 1:100 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules HepG2
• Résultats positifs en IP: cellules HepG2
• Résultats positifs en IHC: tissu ovarien humain, tissu cérébral humain, tissu placentaire humain, tissu pulmonaire humain, tissu splénique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Informations sur le produit

17254-1-AP cible BBX dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, poisson-zèbre
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: BBX Protéine recombinante Ag10691
• Nom complet: bobby sox homolog (Drosophila)
• Masse moléculaire calculée: 941 aa, 105 kDa
• Poids moléculaire observé: 105 kDa
• Numéro d’acquisition GenBank: BC012837
• Symbole du gène: BBX
• Identification du gène (NCBI): 56987
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

BBX, also named HBP2, is a protein with a high mobility group (HMG) domain that belongs to the Sox (Sry-related HMG box) family of transcription factors. As a DNA-binding domain in HBP2, HMG is essential for its function in Schizosaccharomyces pombe. HBP2 activates CdcIO-dependent transcription at G1/s and allows growth of cdc10-129, resldelta and rep2delta mutant cells at the restrictive temperature

Publications

Species	Application	Title
rat	WB,IHC,IF	Biol Reprod; PiggyBac Transposon-Mediated Mutagenesis in Rats Reveals a Crucial Role of Bbx in Growth and Male Fertility.; Authors - Chieh-Ying Wang ; KO Validated
zebrafish	IHC	Dev Genes Evol; Characterization of Bbx, a member of a novel subfamily of the HMG-box superfamily together with Cic.; Authors - TieLin Chen
	WB	Dev Comp Immunol; Discovery of BbX transcription factor in the patagonian blennie: Exploring expression changes following combined bacterial and thermal stress exposure; Authors - Danixa Martínez