Anticorps Polyclonal de lapin anti-BCAT1

• Phare
• Validé par KD/KO

BCAT1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

N° de cat : 13640-1-PBS

Synonymes

PNAS 121, MECA39, ECA39, EC:2.6.1.42, Branched-chain-amino-acid aminotransferase, cytosolic

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Galerie de données de validation

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  WB analysis of HeLa using 13640-1-AP (same clone as 13640-1-PBS)

  WB result of BCAT1/ECA39 antibody (13640-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-BCAT1/ECA39 transfected HeLa cells. This data was developed using the same antibody clone with 13640-1-PBS in a different storage buffer formulation.

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  WB analysis using 13640-1-AP (same clone as 13640-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 13640-1-AP (BCAT1/ECA39 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13640-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 13640-1-AP (same clone as 13640-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13640-1-AP (BCAT1/ECA39 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13640-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 13640-1-AP (same clone as 13640-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 13640-1-AP (BCAT1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13640-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 13640-1-AP (same clone as 13640-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using BCAT1 antibody (13640-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 13640-1-PBS in a different storage buffer formulation.

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Informations sur le produit

13640-1-PBS cible BCAT1 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: BCAT1 Protéine recombinante Ag4574
• Nom complet: branched chain aminotransferase 1, cytosolic
• Masse moléculaire calculée: 320 aa, 36 kDa
• Poids moléculaire observé: 43-45 kDa
• Numéro d’acquisition GenBank: BC033864
• Symbole du gène: BCAT1
• Identification du gène (NCBI): 586
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

BCAT1, also named branched-chain amino acid trasaminase1, is a cytosolic enzyme responsible for the reversible transamination of leucine, isoleucine, and valine that catalyzes the transformation of branched-chain L-amino acids (BCAA) into branched-chain a-ketoacids (BCKA), thereby providing macromolecule precursors. It has been reported that BCAT1 is associated with tumor growth and disease progression (PMID:29255149). The 13640-1-AP antibody detects the bands around 43-45 kDa for different isoforms and higher molecular weight for phosphorylation modification of this protein.

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