Anticorps Monoclonal anti-BCAT1

BCAT1 Monoclonal Antibody for WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

1G3A10

N° de cat : 67084-1-Ig

Synonymes

BCAT1, DKFZp686E12175, ECA39, MECA39, PNAS 121, PP18, Protein ECA39

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Galerie de données de validation

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  WB analysis of Ramos using 67084-1-Ig

  Ramos cells were subjected to SDS PAGE followed by western blot with 67084-1-Ig (BCAT1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of pig brain using 67084-1-Ig

  pig brain tissue were subjected to SDS PAGE followed by western blot with 67084-1-Ig (BCAT1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Daudi using 67084-1-Ig

  Daudi cells were subjected to SDS PAGE followed by western blot with 67084-1-Ig (BCAT1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules Ramos, cellules Daudi, tissu cérébral de porc

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

67084-1-Ig cible BCAT1 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, porc

• Réactivité: Humain, porc
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: BCAT1 Protéine recombinante Ag28328
• Nom complet: branched chain aminotransferase 1, cytosolic
• Masse moléculaire calculée: 320 aa, 36 kDa
• Poids moléculaire observé: 42-45 kDa
• Numéro d’acquisition GenBank: BC033864
• Symbole du gène: BCAT1
• Identification du gène (NCBI): 586
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20 ℃. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

BCAT1, also named as branched-chain amino acid trasaminase1, is a cytosolic enzyme responsible for the reversible transamination of leucine, isoleucine and valine that catalyzes the transformation of branched-chain L-amino acids (BCAA) into branched-chain a-ketoacids (BCKA), thereby providing macromolecule precursors. It has been reported that BCAT1 is associated with tumor growth and disease progression (PMID:29255149).

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