Anticorps Monoclonal anti-BCAT2

BCAT2 Monoclonal Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, souris

Applications

WB, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1A5A5

N° de cat : 68586-1-PBS

Synonymes

PP18, Placental protein 18, ECA40, EC:2.6.1.42, Branched-chain-amino-acid aminotransferase, mitochondrial

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Galerie de données de validation

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  WB analysis using 68586-1-Ig (same clone as 68586-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68586-1-Ig (BCAT2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 68586-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 68586-1-Ig (same clone as 68586-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using BCAT2 antibody (68586-1-Ig, Clone: 1A5A5 ) at dilution of 1:1130 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 68586-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP50268-2

  Cytometric bead array standard curve of MP50268-2, BCAT2 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68586-1-PBS. Detection antibody: 68586-4-PBS. Standard:Ag9595. Range: 0.781-100 ng/mL.

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Informations sur le produit

68586-1-PBS cible BCAT2 dans les applications de WB, IF/ICC, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: BCAT2 Protéine recombinante Ag9595
• Nom complet: branched chain aminotransferase 2, mitochondrial
• Masse moléculaire calculée: 44 kDa
• Poids moléculaire observé: 38 kDa
• Numéro d’acquisition GenBank: BC001900
• Symbole du gène: BCAT2
• Identification du gène (NCBI): 587
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Branched-chain amino acid transaminase 2 (BCAT2) is an important enzyme in branched-chain amino acids (BCAAs) catabolism that reversibly catalyzes the initial step of BCAA degradation to branched-chain acyl-CoA. In humans, BCAT2 encodes a mitochondrial protein that is ubiquitously expressed in all organs (except hepatocytes) (PMID:32467562). Moreover, other reports showed that overexpressed BCAT2 in pancreatic ductal adenocarcinoma (PDAC) cells enhanced cell proliferation and survival in vitro and in vivo (PMID:35663242).

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