Anticorps Recombinant de lapin anti-BCAT2

BCAT2 Recombinant Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

251057E6

N° de cat : 86367-1-PBS

Synonymes

Branched-chain-amino-acid aminotransferase, mitochondrial, EC:2.6.1.42, ECA40, Placental protein 18, PP18

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Galerie de données de validation

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  WB analysis using 86367-1-RR (same clone as 86367-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86367-1-RR (BCAT2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86367-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 86367-1-RR (same clone as 86367-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 86367-1-RR (BCAT2 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86367-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 86367-1-RR (same clone as 86367-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 86367-1-RR (BCAT2 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86367-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 86367-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86367-1-RR against Human BCAT2 were performed. The affinity constant is below 1 pM.

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Informations sur le produit

86367-1-PBS cible BCAT2 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: BCAT2 Protéine recombinante Ag9573
• Nom complet: branched chain aminotransferase 2, mitochondrial
• Masse moléculaire calculée: 44 kDa
• Poids moléculaire observé: 38 kDa
• Numéro d’acquisition GenBank: BC001900
• Symbole du gène: BCAT2
• Identification du gène (NCBI): 587
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Branched-chain amino acid transaminase 2 (BCAT2) is an important enzyme in branched-chain amino acids (BCAAs) catabolism that reversibly catalyzes the initial step of BCAA degradation to branched-chain acyl-CoA. In humans, BCAT2 encodes a mitochondrial protein that is ubiquitously expressed in all organs (except hepatocytes) (PMID:32467562). Moreover, other reports showed that overexpressed BCAT2 in pancreatic ductal adenocarcinoma (PDAC) cells enhanced cell proliferation and survival in vitro and in vivo (PMID:35663242).

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