Anticorps Polyclonal de lapin anti-BCCIP

• Phare
• Validé par KD/KO

BCCIP Polyclonal Antibody for WB, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 16043-1-PBS

Synonymes

TOK1, TOK 1, Protein TOK-1, Protein TOK 1, P21- and CDK-associated protein 1

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Galerie de données de validation

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  WB analysis of HeLa using 16043-1-AP (same clone as 16043-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 16043-1-AP (BCCIP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16043-1-PBS in a different storage buffer formulation.

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  WB analysis of A549 using 16043-1-AP (same clone as 16043-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 16043-1-AP (BCCIP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16043-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 16043-1-AP (same clone as 16043-1-PBS)

  IP result of anti-BCCIP (IP:16043-1-AP, 4ug; Detection:16043-1-AP 1:800) with HeLa cells lysate 3200ug. This data was developed using the same antibody clone with 16043-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 16043-1-AP (same clone as 16043-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using BCCIP antibody (16043-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 16043-1-PBS in a different storage buffer formulation.

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Informations sur le produit

16043-1-PBS cible BCCIP dans les applications de WB, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: BCCIP Protéine recombinante Ag8934
• Nom complet: BRCA2 and CDKN1A interacting protein
• Masse moléculaire calculée: 314 aa, 36 kDa
• Poids moléculaire observé: 50-58 kDa
• Numéro d’acquisition GenBank: BC009771
• Symbole du gène: BCCIP
• Identification du gène (NCBI): 56647
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

BCCIP (or TOK1) is isolated as a BRCA2- and CDKN1A (p21)-interacting protein and is implicated in homologous recombination (HR) pathway and inhibition of DNA replication stress. BCCIP is an evolutionarily conserved nuclear protein with multiple interacting domains. BCCIP deficiency in mice impaired embryonic and postnatal neural development, causing severe ataxia, cerebral and cerebellar defects, and microcephaly, which are associated with spontaneous DNA damage and subsequent cell death in the proliferative cell populations of the neural system during embryogenesis. BCCIP is essential for maintaining the transactivation activity of wild type p53 suggesting a potential role of BCCIP in cancer etiology.

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