Anticorps Polyclonal de lapin anti-BCKDK

• Phare
• Validé par KD/KO

BCKDK Polyclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 15718-1-PBS

Synonymes

[3-methyl-2-oxobutanoate dehydrogenase [lipoamide]] kinase, mitochondrial, BCKDH kinase, BCKDHKIN, BDK, Branched-chain alpha-ketoacid dehydrogenase kinase

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Galerie de données de validation

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  WB analysis of mouse pancreas using 15718-1-AP (same clone as 15718-1-PBS)

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 15718-1-AP (BCKDK antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15718-1-PBS in a different storage buffer formulation.

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  WB analysis using 15718-1-AP (same clone as 15718-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 15718-1-AP (BCKDK antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15718-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 15718-1-AP (same clone as 15718-1-PBS)

  WB result of BCKDK antibody (15718-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-BCKDK transfected HEK-293 cells. This data was developed using the same antibody clone with 15718-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse pancreas using 15718-1-AP (same clone as 15718-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 15718-1-AP (BCKDK antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15718-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse pancreas using 15718-1-AP (same clone as 15718-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 15718-1-AP (BCKDK antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15718-1-PBS in a different storage buffer formulation.

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Informations sur le produit

15718-1-PBS cible BCKDK dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: BCKDK Protéine recombinante Ag8395
• Nom complet: branched chain ketoacid dehydrogenase kinase
• Masse moléculaire calculée: 412 aa, 46 kDa
• Poids moléculaire observé: 40 kDa
• Numéro d’acquisition GenBank: BC007363
• Symbole du gène: BCKDK
• Identification du gène (NCBI): 10295
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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