Anticorps Monoclonal anti-BCL10

BCL10 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2E8G5

N° de cat : 66556-1-PBS

Synonymes

2E8G5, B-cell CLL/lymphoma 10, B-cell lymphoma/leukemia 10, Bcl 10, Bcl-10

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Galerie de données de validation

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  WB analysis using 66556-1-Ig (same clone as 66556-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66556-1-Ig (BCL10 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 66556-1-PBS in a different storage buffer formulation.

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  WB analysis of Raji using 66556-1-Ig (same clone as 66556-1-PBS)

  Raji, K-562, and HL-60 cells were subjected to SDS PAGE followed by western blot with 66556-1-Ig (BCL10 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66556-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 66556-1-Ig (same clone as 66556-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66556-1-Ig (BCL10 antibody) at dilution of 1:700 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66556-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 66556-1-Ig (same clone as 66556-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66556-1-Ig (BCL10 antibody) at dilution of 1:700 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66556-1-PBS in a different storage buffer formulation.

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  IHC staining of human lymphoma using 66556-1-Ig (same clone as 66556-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66556-1-Ig (BCL10 antibody) at dilution of 1:700 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66556-1-PBS in a different storage buffer formulation.

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  IHC staining of human lymphoma using 66556-1-Ig (same clone as 66556-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66556-1-Ig (BCL10 antibody) at dilution of 1:700 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66556-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66556-1-Ig (same clone as 66556-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using BCL10 antibody (66556-1-Ig, Clone: 2E8G5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66556-1-PBS in a different storage buffer formulation.

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  FC experiment of Jurkat using 66556-1-Ig (same clone as 66556-1-PBS)

  1x10^6 Jurkat cells were intracellularly stained with 0.4 ug BCL10 Monoclonal antibody (66556-1-Ig, Clone:2E8G5) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66556-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66556-1-PBS cible BCL10 dans les applications de WB, IHC, IF/ICC, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: BCL10 Protéine recombinante Ag12162
• Nom complet: B-cell CLL/lymphoma 10
• Masse moléculaire calculée: 233 aa, 26 kDa
• Poids moléculaire observé: 30-32 kDa
• Numéro d’acquisition GenBank: BC053617
• Symbole du gène: BCL10
• Identification du gène (NCBI): 8915
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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