Anticorps Recombinant de lapin anti-BCL10

BCL10 Recombinant Antibody for WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

250149A6

N° de cat : 85858-2-PBS

Synonymes

B-cell CLL/lymphoma 10, B-cell lymphoma/leukemia 10, Bcl 10, Bcl-10, c E10

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Galerie de données de validation

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  WB analysis using 85858-2-RR (same clone as 85858-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85858-2-RR (BCL10 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85858-2-PBS in a different storage buffer formulation.

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  Sandwich ELISA sample test of MP02160-2

  The mean BCL10 concentration was determined to be 105.67 ng/mL in Jurkat cell extract based on a 1.20 mg/mL extract load, 327.67 ng/mL in K-562 cell extract based on a 3.00 mg/mL extract load and 82.11 ng/mL in A549 cell extract based on a 1.10 mg/mL extract load.

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  Sandwich ELISA standard curve of MP02160-2

  Sandwich ELISA standard curve of MP02160-2, Human BCL10 Recombinant Matched Antibody Pair - PBS only. 85858-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag12170. 85858-2-PBS was HRP conjugated as the detection antibody. Range: 0.313-20 ng/mL.

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  Cytometric bead array standard curve of MP02160-2

  Cytometric bead array standard curve of MP02160-2, BCL10 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85858-3-PBS. Detection antibody: 85858-2-PBS. Standard: Ag12170. Range: 0.781-100 ng/mL.

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  Affinity and Kinetic Characterization of 85858-2-RR

  Biolayer interferometry (BLl) kinetic assays of 85858-2-RR against Human BCL10 were performed. The affinity constant is below 1 pM.

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Informations sur le produit

85858-2-PBS cible BCL10 dans les applications de WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: BCL10 Protéine recombinante Ag12170
• Nom complet: B-cell CLL/lymphoma 10
• Masse moléculaire calculée: 233 aa, 26 kDa
• Poids moléculaire observé: 30 kDa
• Numéro d’acquisition GenBank: BC053617
• Symbole du gène: BCL10
• Identification du gène (NCBI): 8915
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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