Anticorps Polyclonal de lapin anti-BCL11B

BCL11B Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 55414-1-PBS

Synonymes

COUP TF interacting protein 2, BCL-11B, B-cell lymphoma/leukemia 11B, B-cell CLL/lymphoma 11B, B cell lymphoma/leukemia 11B

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Galerie de données de validation

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  WB analysis of Jurkat using 55414-1-AP (same clone as 55414-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 55414-1-AP (BCL11B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55414-1-PBS in a different storage buffer formulation.

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  WB analysis using 55414-1-AP (same clone as 55414-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 55414-1-AP (BCL11B antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55414-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse thymus using 55414-1-AP (same clone as 55414-1-PBS)

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 55414-1-AP (BCL11B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55414-1-PBS in a different storage buffer formulation.

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  IP experiment of Jurkat using 55414-1-AP (same clone as 55414-1-PBS)

  IP result of anti-BCL11B (IP:55414-1-AP, 4ug; Detection:55414-1-AP 1:5000) with Jurkat cells lysate 1320 ug. This data was developed using the same antibody clone with 55414-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 55414-1-AP (same clone as 55414-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 55414-1-AP (BCL11B antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55414-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 55414-1-AP (same clone as 55414-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 55414-1-AP (BCL11B antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 55414-1-PBS in a different storage buffer formulation.

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Informations sur le produit

55414-1-PBS cible BCL11B dans les applications de WB, IHC, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: B-cell CLL/lymphoma 11B (zinc finger protein)
• Masse moléculaire calculée: 96 kDa
• Poids moléculaire observé: 116 kDa
• Numéro d’acquisition GenBank: NM_022898
• Symbole du gène: BCL11B
• Identification du gène (NCBI): 64919
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

BCL11B, also named CTIP2, RIT1, CTIP-2, ZNF856B, and hRIT1-alpha, is a tumor-suppressor protein involved in T-cell lymphomas. It may function on the P53-signaling pathway. BCL11B is a key regulator of both differentiation and survival during thymocyte development. It depresses transcription through direct, TFCOUP2-independent binding to a GC-rich response element. This antibody is specific to BCL11B. The calculated molecular weight of BCL11B is 95 kDa but the modified BCL11B protein is about 116 kDa.

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