Anticorps Recombinant de lapin anti-BCL11B

BCL11B Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

242683B9

N° de cat : 85363-1-RR

Synonymes

242683B9, B cell CLL/lymphoma 11B, B cell lymphoma/leukemia 11B, B-cell CLL/lymphoma 11B, B-cell lymphoma/leukemia 11B

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Galerie de données de validation

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  WB analysis using 85363-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85363-1-RR (BCL11B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IF Staining of Jurkat using 85363-1-RR

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using BCL11B antibody (85363-1-RR, Clone: 242683B9 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  FC experiment of Jurkat using 85363-1-RR

  1x10^6 Jurkat cells were intracellularly stained with 0.25 ug BCL11B Recombinant antibody (85363-1-RR, Clone:242683B9) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules MOLT-4, tissu de thymus de souris
• Résultats positifs en IF/ICC: cellules Jurkat,
• Résultats positifs en FC (Intra): cellules Jurkat,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

85363-1-RR cible BCL11B dans les applications de WB, IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: B-cell CLL/lymphoma 11B (zinc finger protein)
• Masse moléculaire calculée: 96 kDa
• Poids moléculaire observé: 116 kDa
• Numéro d’acquisition GenBank: NM_022898
• Symbole du gène: BCL11B
• Identification du gène (NCBI): 64919
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

BCL11B, also named CTIP2, RIT1, CTIP-2, ZNF856B, and hRIT1-alpha, is a tumor-suppressor protein involved in T-cell lymphomas. It may function on the P53-signaling pathway. BCL11B is a key regulator of both differentiation and survival during thymocyte development. It depresses transcription through direct, TFCOUP2-independent binding to a GC-rich response element. This antibody is specific to BCL11B. The calculated molecular weight of BCL11B is 95 kDa but the modified BCL11B protein is about 116 kDa.

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