Anticorps Polyclonal de lapin anti-BCL2L14

BCL2L14 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

N° de cat : 12846-1-PBS

Synonymes

---

Galerie de données de validation

• 

  WB analysis of A549 using 12846-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 12846-1-AP (BCL2L14 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human lung using 12846-1-AP (same clone as 12846-1-PBS)

  human lung tissue were subjected to SDS PAGE followed by western blot with 12846-1-AP (BCL2L14 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12846-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 12846-1-AP (same clone as 12846-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 12846-1-AP (BCL2L14 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 12846-1-PBS in a different storage buffer formulation.

• 

  IF Staining of MCF-7 using 12846-1-AP (same clone as 12846-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using 12846-1-AP (BCL2L14 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12846-1-PBS in a different storage buffer formulation.

• 

  FC experiment of Jurkat using 12846-1-AP (same clone as 12846-1-PBS)

  1x10^6 Jurkat cells were intracellularly stained with 0.4 ug BCL2L14 Polyclonal antibody (12846-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 12846-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

12846-1-PBS cible BCL2L14 dans les applications de WB, IHC, IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: BCL2L14 Protéine recombinante Ag3926
• Nom complet: BCL2-like 14 (apoptosis facilitator)
• Masse moléculaire calculée: 327 aa, 37 kDa
• Poids moléculaire observé: 37 kDa
• Numéro d’acquisition GenBank: BC025778
• Symbole du gène: BCL2L14
• Identification du gène (NCBI): 79370
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

{{ptg:RelatedPrimaryAntibodies}}