Anticorps Polyclonal de lapin anti-BCL3

• Phare
• Validé par KD/KO

BCL3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, IP, CoIP, chIP, ELISA

Conjugaison

Non conjugué

Publications(13)

Avis (2)

N° de cat : 23959-1-AP

Synonymes

B-cell lymphoma 3 protein, BCL-3, BCL4, D19S37, Proto-oncogene BCL3

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Galerie de données de validation

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  WB analysis of HeLa using 23959-1-AP

  WB result of BCL3 antibody (23959-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-BCL3 transfected HeLa cells.

• 

  WB analysis of HEK-293 using 23959-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 23959-1-AP (BCL3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 23959-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 23959-1-AP (BCL3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 23959-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 23959-1-AP (BCL3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 23959-1-AP

  IP result of anti-BCL3 (IP:23959-1-AP, 4ug; Detection:23959-1-AP 1:600) with HEK-293 cells lysate 3000ug.

• 

  IHC staining of human lymphoma using 23959-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 23959-1-AP (BCL3 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse spleen using 23959-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 23959-1-AP (BCL3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse spleen using 23959-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 23959-1-AP (BCL3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 23959-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 23959-1-AP (BCL3 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lymphoma using 23959-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 23959-1-AP (BCL3 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lymphoma using 23959-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 23959-1-AP (BCL3 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 23959-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 23959-1-AP (BCL3 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 23959-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 23959-1-AP (BCL3 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 23959-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using BCL3 antibody (23959-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules A549, cellules HeLa
• Résultats positifs en IP: cellules HEK-293
• Résultats positifs en IHC: tissu de lymphome humain, tissu splénique de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 9 publications below
• IHC: See 4 publications below
• IF: See 1 publications below
• IP: See 3 publications below
• CoIP: See 2 publications below
• ChIP: See 1 publications below

Informations sur le produit

23959-1-AP cible BCL3 dans les applications de WB, IHC, IF/ICC, IP, CoIP, chIP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: BCL3 Protéine recombinante Ag21040
• Nom complet: B-cell CLL/lymphoma 3
• Masse moléculaire calculée: 446 aa, 47 kDa
• Poids moléculaire observé: 48-60 kDa
• Numéro d’acquisition GenBank: BC064993
• Symbole du gène: BCL3
• Identification du gène (NCBI): 602
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

BCL3 (B-cell lymphoma 3 protein) has seven ankyrin repeat domains, a proline-rich N terminal domain and a serine- and proline-rich C-terminal domain(PMID: 31930327). BCL3 is an important contributor to chronic inflammatory disease states, such as osteoarthritis and rheumatoid arthritis (PMID: 12483727). BCL3 is a predominantly nuclear protein containing a trans-activation domain, and it can be recruited to NF-κB-responsive promoters, resulting in transcriptional activation or repression depending on the subunit composition of NF-κB complexes (PMID: 25089799, PMID: 30135206). BCL3 is known to be overexpressed in human breast cancers and is associated with reduced metastasis-free survival (PMID: 38255248).

Publications

Species	Application	Title
mouse	IHC	Nature; Divergent transcriptional regulation of astrocyte reactivity across disorders.; Authors - Joshua E Burda
human	IHC	Nucleic Acids Res; SPaRTAN, a computational framework for linking cell-surface receptors to transcriptional regulators.; Authors - Xiaojun Ma
human	WB,IP	Mol Cancer Ther; The Discovery of a Novel Antimetastatic Bcl3 Inhibitor.; Authors - Jitka Soukupová
human	WB	Carcinogenesis; Bcl-3 promotes multi-modal tumour cell migration via NF-κB1 mediated regulation of Cdc42.; Authors - Daniel J Turnham ; KD Validated
human	WB,chIP	J Biol Chem; The proto-oncogene Bcl3 induces immune checkpoint PD-L1 expression, mediating proliferation of ovarian cancer cells.; Authors - Yue Zou ; KD Validated
human	WB	Int J Oncol; BCL‑3 promotes cyclooxygenase‑2/prostaglandin E2 signalling in colorectal cancer.; Authors - Tracey Jane Collard

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Udesh (Verified Customer) (08-16-2023)

Worked well for WB and IF

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1:2000 for WB and 1:200 for IF
• Cell Tissue Type: Bone Marrow MSCs

FH

Ewelina (Verified Customer) (07-03-2019)

This is the only antibody for Western Blot, that worked for me for the detection of endogenous BCL3 in human lymphoma cell lines. It works perfectly.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: human lymphoma cell lines WT and KO

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