Anticorps Monoclonal anti-BCL6

BCL6 Monoclonal Antibody for FC, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

6

CloneNo.

1E6B1

N° de cat : 66340-1-Ig

Synonymes

B cell CLL/lymphoma 6, B cell lymphoma 5 protein, B cell lymphoma 6 protein, BCL6, LAZ3, Protein LAZ 3, ZBTB27, Zinc finger protein 51, ZNF51

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Galerie de données de validation

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  WB analysis of Daudi using 66340-1-Ig

  Daudi cells were subjected to SDS PAGE followed by western blot with 66340-1-Ig (BCL6 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Ramos using 66340-1-Ig

  Ramos cells were subjected to SDS PAGE followed by western blot with 66340-1-Ig (BCL6 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 66340-1-Ig

  Raji cells were subjected to SDS PAGE followed by western blot with 66340-1-Ig (BCL6 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human tonsillitis using 66340-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66340-1-Ig (BCL6 Antibody) at dilution of 1:1000 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH9). The signal was enhanced by soaking the slide in 1% CuSO4 for 10 minutes after DAB development.

• 

  IHC staining of human tonsillitis using 66340-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66340-1-Ig (BCL6 Antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieved with Tris-EDTA buffer (pH9).The signal was enhanced by soaking the slide in 1% CuSO4 for 10 minutes after DAB development.

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  FC experiment of Jurkat using 66340-1-Ig

  1X10^6 Jurkat cells were intracellularly stained with 0.2 ug Anti-Human BCL6 (66340-1-Ig, Clone:1E6B1) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Mouse IgG1 Isotype Control (66360-1-Ig, Clone: T1F8D3F10) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Applications testées

• Résultats positifs en WB: cellules Daudi, cellules Raji, cellules Ramos
• Résultats positifs en IHC: tissu d'amygdalite humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en cytométrie: cellules Jurkat,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:1000-1:2000
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IHC: See 5 publications below
• IF: See 2 publications below

Informations sur le produit

66340-1-Ig cible BCL6 dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: BCL6 Protéine recombinante Ag15519
• Nom complet: B-cell CLL/lymphoma 6
• Masse moléculaire calculée: 706 aa, 79 kDa
• Poids moléculaire observé: 85 kDa
• Numéro d’acquisition GenBank: BC150184
• Symbole du gène: BCL6
• Identification du gène (NCBI): 604
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

BCL6, a zinc finger transcription factor, contains an N-terminal BTB/POZ domain and C-terminal zinc finger DNA-binding motifs and represses transcription of a wide range of target proteins and microRNAs. BCL6 protein has been reported as a master regulator of B lymphocyte development and growth, and altered BCL6 protein expression was implicated in pathogenesis of diverse human hematologic malignancies, especially in the diffuse large B cell lymphoma (DLBCL). BCL6 is required for the development of T follicular helper T cells (TFH), a helper T cell subset required for the formation of mature and productive GCs. BCL6 has also been shown to play important regulatory roles in macrophages. This antibody only recognize human species.

Publications

Species	Application	Title
human	IF	Nat Commun; B cells sustain inflammation and predict response to immune checkpoint blockade in human melanoma.; Authors - Johannes Griss
human	IHC	Clin Transl Med; Single-cell transcriptome sequencing of B-cell heterogeneity and tertiary lymphoid structure predicts breast cancer prognosis and neoadjuvant therapy efficacy; Authors - Qing Wang
human	IHC，IF	Front Immunol; A Standardized Analysis of Tertiary Lymphoid Structures in Human Melanoma: Disease Progression- and Tumor Site-Associated Changes With Germinal Center Alteration.; Authors - Franziska Werner
human	IHC	iScience; Tertiary lymphoid structure and decreased CD8+ T cell infiltration in minimally invasive adenocarcinoma.; Authors - Jin Wang
mouse	IHC	Cell Death Dis; B-cell lymphoma 6 alleviates nonalcoholic fatty liver disease in mice through suppression of fatty acid transporter CD36.; Authors - Hao Zhang
human	IHC	Front Oncol; Clinical Impact of the Histopathological Index and Neuroimaging Features Status in Primary Central Nervous System Diffuse Large B-Cell Lymphoma: A Single-Center Retrospective Analysis of 51 Cases.; Authors - Zhou Qi

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