Anticorps Monoclonal anti-BCL6

BCL6 Monoclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1E6B1

N° de cat : 66340-1-PBS

Synonymes

BCL-6, 1E6B1, B cell CLL/lymphoma 6, B cell lymphoma 6 protein, B-cell lymphoma 5 protein

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Galerie de données de validation

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  WB analysis of Ramos using 66340-1-Ig (same clone as 66340-1-PBS)

  Ramos cells were subjected to SDS PAGE followed by western blot with 66340-1-Ig (BCL6 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66340-1-PBS in a different storage buffer formulation.

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  WB analysis of Daudi using 66340-1-Ig (same clone as 66340-1-PBS)

  Daudi cells were subjected to SDS PAGE followed by western blot with 66340-1-Ig (BCL6 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66340-1-PBS in a different storage buffer formulation.

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  WB analysis of Raji using 66340-1-Ig (same clone as 66340-1-PBS)

  Raji cells were subjected to SDS PAGE followed by western blot with 66340-1-Ig (BCL6 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66340-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 66340-1-Ig (same clone as 66340-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66340-1-Ig (BCL6 antibody) at dilution of 1:4000 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66340-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 66340-1-Ig (same clone as 66340-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66340-1-Ig (BCL6 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66340-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66340-1-PBS cible BCL6 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: BCL6 Protéine recombinante Ag15519
• Nom complet: B-cell CLL/lymphoma 6
• Masse moléculaire calculée: 706 aa, 79 kDa
• Poids moléculaire observé: 85 kDa
• Numéro d’acquisition GenBank: BC150184
• Symbole du gène: BCL6
• Identification du gène (NCBI): 604
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

BCL6, a zinc finger transcription factor, contains an N-terminal BTB/POZ domain and C-terminal zinc finger DNA-binding motifs and represses transcription of a wide range of target proteins and microRNAs. BCL6 protein has been reported as a master regulator of B lymphocyte development and growth, and altered BCL6 protein expression was implicated in pathogenesis of diverse human hematologic malignancies, especially in the diffuse large B cell lymphoma (DLBCL). BCL6 is required for the development of T follicular helper T cells (TFH), a helper T cell subset required for the formation of mature and productive GCs. BCL6 has also been shown to play important regulatory roles in macrophages.

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