Anticorps Recombinant de lapin anti-BCL6

• Phare
• Validé par KD/KO

BCL6 Recombinant Antibody for WB, IHC, FC (Intra), IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, FC (Intra), IP, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

3K6

N° de cat : 81754-1-PBS

Synonymes

BCL-6, 3K6, B cell CLL/lymphoma 6, B cell lymphoma 6 protein, B-cell lymphoma 5 protein

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Galerie de données de validation

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  WB analysis of Daudi using 81754-1-RR (same clone as 81754-1-PBS)

  WB result of BCL6 antibody (81754-1-RR; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-BCL6 transfected Daudi cells. This data was developed using the same antibody clone with 81754-1-PBS in a different storage buffer formulation.

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  WB analysis using 81754-1-RR (same clone as 81754-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 81754-1-RR (BCL6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81754-1-PBS in a different storage buffer formulation.

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  WB analysis of A20 using 81754-1-RR (same clone as 81754-1-PBS)

  A20 cells were subjected to SDS PAGE followed by western blot with 81754-1-RR (BCL6 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81754-1-PBS in a different storage buffer formulation.

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  IP experiment of Ramos using 81754-1-RR (same clone as 81754-1-PBS)

  IP result of anti-BCL6 (IP:81754-1-RR, 4ug; Detection:81754-1-RR 1:8000) with Ramos cells lysate 1680 ug. This data was developed using the same antibody clone with 81754-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 81754-1-RR (same clone as 81754-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 81754-1-RR (BCL6 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81754-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 81754-1-RR (same clone as 81754-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 81754-1-RR (BCL6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81754-1-PBS in a different storage buffer formulation.

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  FC experiment of Ramos using 81754-1-RR (same clone as 81754-1-PBS)

  1X10^6 Ramos cells were intracellularly stained with 0.4 ug Anti-Human BCL6 (81754-1-RR, Clone:3K6) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 81754-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 81754-1-RR

  Biolayer interferometry (BLl) kinetic assays of 81754-1-RR against Human BCL6 were performed. The affinity constant is 0.157 nM.

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Informations sur le produit

81754-1-PBS cible BCL6 dans les applications de WB, IHC, FC (Intra), IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: BCL6 Protéine recombinante Ag15519
• Nom complet: B-cell CLL/lymphoma 6
• Masse moléculaire calculée: 706 aa, 79 kDa
• Poids moléculaire observé: 79-95 kDa
• Numéro d’acquisition GenBank: BC150184
• Symbole du gène: BCL6
• Identification du gène (NCBI): 604
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

BCL6, a zinc finger transcription factor, contains an N-terminal BTB/POZ domain and C-terminal zinc finger DNA-binding motifs and represses transcription of a wide range of target proteins and microRNAs. BCL6 protein has been reported as a master regulator of B lymphocyte development and growth, and altered BCL6 protein expression was implicated in pathogenesis of diverse human hematologic malignancies, especially in the diffuse large B cell lymphoma (DLBCL). BCL6 is required for the development of T follicular helper T cells (TFH), a helper T cell subset required for the formation of mature and productive GCs. BCL6 has also been shown to play important regulatory roles in macrophages.

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