Anticorps Recombinant de lapin anti-BCL9

BCL9 Recombinant Antibody for IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

240682A9

N° de cat : 83760-3-PBS

Synonymes

Bcl-9, 240682A9, B cell CLL/lymphoma 9, B-cell CLL/lymphoma 9 protein, B-cell lymphoma 9 protein

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Galerie de données de validation

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  IF Staining of HeLa using 83760-3-RR (same clone as 83760-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using BCL9 antibody (83760-3-RR, Clone: 240682A9 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83760-3-PBS in a different storage buffer formulation.

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  FC experiment of SH-SY5Y using 83760-3-RR (same clone as 83760-3-PBS)

  1x10^6 SH-SY5Y cells were intracellularly stained with 0.25 ug Bcl9 Recombinant Antibody (83760-3-RR, Clone:240682A9) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 83760-3-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83760-3-PBS

  Biolayer interferometry (BLl) kinetic assay of 83760-3-PBS against Human BCL9 was performed. The affinity constant is 5.40 nM.

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  Affinity and Kinetic Characterization of 83760-3-RR

  Biolayer interferometry (BLl) kinetic assays of 83760-3-RR against Human BCL9 were performed. The affinity constant is 5.39 nM.

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Informations sur le produit

83760-3-PBS cible BCL9 dans les applications de IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: BCL9 Protéine recombinante Ag19158
• Nom complet: B-cell CLL/lymphoma 9
• Masse moléculaire calculée: 1426 aa, 149 kDa
• Numéro d’acquisition GenBank: BC116451
• Symbole du gène: BCL9
• Identification du gène (NCBI): 607
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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