Anticorps Recombinant de lapin anti-BCRP/ABCG2

• Phare
• Validé par KD/KO

BCRP/ABCG2 Recombinant Antibody for WB, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

240959A4

N° de cat : 83835-1-PBS

Synonymes

ABCG2, BCRP, BCRP,ABCG2, 240959A4, ABC15

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Galerie de données de validation

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  WB analysis using 83835-1-RR (same clone as 83835-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83835-1-RR (BCRP,ABCG2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83835-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 83835-1-RR (same clone as 83835-1-PBS)

  WB result of BCRP/ABCG2 antibody (83835-1-RR; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-BCRP/ABCG2 transfected HEK-293 cells. This data was developed using the same antibody clone with 83835-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00805-1

  Cytometric bead array standard curve of MP00805-1, BCRP/ABCG2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83835-1-PBS. Detection antibody: 83835-2-PBS. Standard: Ag25911. Range: 0.625-80 ng/mL.

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  Affinity and Kinetic Characterization of 83835-1-PBS

  Biolayer interferometry (BLl) kinetic assay of 83835-1-PBS against Human BCRP/ABCG2 was performed. The affinity constant is 4.49 nM.

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  Affinity and Kinetic Characterization of 83835-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83835-1-RR against Human BCRP,ABCG2 were performed. The affinity constant is 9.85 nM.

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Informations sur le produit

83835-1-PBS cible BCRP/ABCG2 dans les applications de WB, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: BCRP/ABCG2 Protéine recombinante Ag25911
• Nom complet: ATP-binding cassette, sub-family G (WHITE), member 2
• Masse moléculaire calculée: 70 kDa
• Poids moléculaire observé: 68-72 kDa
• Numéro d’acquisition GenBank: BC021281
• Symbole du gène: ABCG2
• Identification du gène (NCBI): 9429
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

BCRP (breast cancer resistance protein) confers a broad spectrum of drug resistance and the expression of BCRP is enhanced in several cancer cell models. It has ATPase activity and belongs to a family of multiple drug resistant proteins. Data also indicated that BCRP might be an early marker for some stem cells.

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