Anticorps Polyclonal de lapin anti-BDH1

• Phare
• Validé par KD/KO

BDH1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

23

N° de cat : 15417-1-AP

Synonymes

BDH, BDH1, SDR9C1

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Galerie de données de validation

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  WB analysis using 15417-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15417-1-AP (BDH1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 15417-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 15417-1-AP (BDH1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 15417-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 15417-1-AP (BDH1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 15417-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 15417-1-AP (BDH1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HT-29 using 15417-1-AP

  HT-29 cells were subjected to SDS PAGE followed by western blot with 15417-1-AP (BDH1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 15417-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 15417-1-AP (BDH1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 15417-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 15417-1-AP (BDH1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 15417-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 15417-1-AP (BDH1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 15417-1-AP

  IP Result of anti-BDH1 (IP:15417-1-AP, 4ug; Detection:15417-1-AP 1:300) with mouse liver tissue lysate 4000ug.

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  IHC staining of rat brain using 15417-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 15417-1-AP (BDH1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 15417-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15417-1-AP (BDH1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 15417-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15417-1-AP (BDH1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 15417-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 15417-1-AP (BDH1 antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules COLO 320, cellules HT-29, tissu cardiaque de souris, tissu cérébral de rat, tissu cérébral de souris, tissu hépatique de rat, tissu hépatique de souris, tissu hépatique humain
• Résultats positifs en IP: tissu hépatique de souris
• Résultats positifs en IHC: tissu cérébral de rat, tissu de cancer du foie humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 20 publications below
• IHC: See 3 publications below
• IF: See 1 publications below

Informations sur le produit

15417-1-AP cible BDH1 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: BDH1 Protéine recombinante Ag7705
• Nom complet: 3-hydroxybutyrate dehydrogenase, type 1
• Masse moléculaire calculée: 38 kDa
• Poids moléculaire observé: 31 kDa
• Numéro d’acquisition GenBank: BC005844
• Symbole du gène: BDH1
• Identification du gène (NCBI): 622
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

BDH1 (3-hydroxybutyrate dehydrogenase, type 1) is a 343 amino acid protein that localizes to the mitochondrial matrix and belongs to the short-chain dehydrogenases/reductases (SDR) family. The deduced 343 amino acid protein contains a 46 residue leader peptide, which is cleaved to produce the mature form. It is a lipid-requiring mitochondrial enzyme with a specific requirement of phosphatidylcholine (PC).

Publications

Species	Application	Title
mouse	WB	Cell Metab; Hepatocyte-Macrophage Acetoacetate Shuttle Protects against Tissue Fibrosis.; Authors - Patrycja Puchalska
human	WB	Cell Res; Hepatocellular carcinoma redirects to ketolysis for progression under nutrition deprivation stress.; Authors - De Huang
mouse	WB	Sci Adv; Ketogenic diet uncovers differential metabolic plasticity of brain cells; Authors - Tim Düking
human	WB	Proc Natl Acad Sci U S A; PMID: 29311302; Authors - Marcus D Goncalves
human,mouse	WB	EMBO Mol Med; Ketone body oxidation increases cardiac endothelial cell proliferation.; Authors - Eva-Maria Weis
mouse	WB	J Cell Biol; CLUH regulates mitochondrial metabolism by controlling translation and decay of target mRNAs.; Authors - Désirée Schatton