Anticorps Monoclonal anti-BDH1

• Phare
• Validé par KD/KO

BDH1 Monoclonal Antibody for WB, IHC, IF-P, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, IF-P, ELISA

Conjugaison

Non conjugué

CloneNo.

2C3D4

N° de cat : 67448-1-PBS

Synonymes

2C3D4, 3-hydroxybutyrate dehydrogenase, BDH, D-beta-hydroxybutyrate dehydrogenase, mitochondrial, EC:1.1.1.30

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Galerie de données de validation

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  WB analysis of pig liver using 67448-1-Ig (same clone as 67448-1-PBS)

  pig liver tissue were subjected to SDS PAGE followed by western blot with 67448-1-Ig (BDH1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67448-1-PBS in a different storage buffer formulation.

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  WB analysis of HSC-T6 using 67448-1-Ig (same clone as 67448-1-PBS)

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67448-1-Ig (BDH1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67448-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 67448-1-Ig (same clone as 67448-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 67448-1-Ig (BDH1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67448-1-PBS in a different storage buffer formulation.

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  WB analysis of HT-29 using 67448-1-Ig (same clone as 67448-1-PBS)

  HT-29 cells were subjected to SDS PAGE followed by western blot with 67448-1-Ig (BDH1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67448-1-PBS in a different storage buffer formulation.

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  WB analysis of COLO 320 using 67448-1-Ig (same clone as 67448-1-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 67448-1-Ig (BDH1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67448-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 67448-1-Ig (same clone as 67448-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67448-1-Ig (BDH1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67448-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 67448-1-Ig (same clone as 67448-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67448-1-Ig (BDH1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67448-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse liver using 67448-1-Ig (same clone as 67448-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using BDH1 antibody (67448-1-Ig, Clone: 2C3D4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67448-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse liver using 67448-1-Ig (same clone as 67448-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using BDH1 antibody (67448-1-Ig, Clone: 2C3D4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67448-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67448-1-PBS cible BDH1 dans les applications de WB, IHC, IF-P, ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: BDH1 Protéine recombinante Ag7864
• Nom complet: 3-hydroxybutyrate dehydrogenase, type 1
• Masse moléculaire calculée: 38 kDa
• Poids moléculaire observé: 31 kDa
• Numéro d’acquisition GenBank: BC005844
• Symbole du gène: BDH1
• Identification du gène (NCBI): 622
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

BDH1 (3-hydroxybutyrate dehydrogenase, type 1) is a 343 amino acid protein that localizes to the mitochondrial matrix and belongs to the short-chain dehydrogenases/reductases (SDR) family. The deduced 343 amino acid protein contains a 46 residue leader peptide, which is cleaved to produce the mature form. It is a lipid-requiring mitochondrial enzyme with a specific requirement of phosphatidylcholine (PC).

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