Anticorps Monoclonal anti-BDH2

• Phare
• Validé par KD/KO

BDH2 Monoclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

1B6H5

N° de cat : 68279-1-PBS

Synonymes

(R)-beta-hydroxybutyrate dehydrogenase, 1B6H5, 3-hydroxybutyrate dehydrogenase type 2, 4-oxo-L-proline reductase, DHRS6

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Galerie de données de validation

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  WB analysis using 68279-1-Ig (same clone as 68279-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68279-1-Ig (BDH2 antibody) at dilution of 1:10000 and incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 68279-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 68279-1-Ig (same clone as 68279-1-PBS)

  WB result of BDH2 antibody (68279-1-Ig; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-BDH2 transfected HeLa cells. This data was developed using the same antibody clone with 68279-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 68279-1-Ig (same clone as 68279-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using BDH2 antibody (68279-1-Ig, Clone: 1B6H5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68279-1-PBS in a different storage buffer formulation.

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Informations sur le produit

68279-1-PBS cible BDH2 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: BDH2 Protéine recombinante Ag26108
• Nom complet: 3-hydroxybutyrate dehydrogenase, type 2
• Poids moléculaire observé: 27 kDa
• Numéro d’acquisition GenBank: BC095414
• Symbole du gène: BDH2
• Identification du gène (NCBI): 56898
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Cytosolic type 2-hydroxybutyrate dehydrogenase, BDH2, originally named DHRS6, plays a physiological role in the cytosolic utilization of ketone bodies, which can subsequently enter mitochondria and the tricarboxylic acid cycle (PMID: 16380372). Moreover, BDH2 catalyzes the production of 2, 3-DHBA during enterobactin biosynthesis and participates in 24p3 (LCN2)-mediated iron transport and apoptosis (PMID: 23150981). It has 3 isoforms with molecular weights of 27, 19, and 16 kDa.

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