Anticorps Polyclonal de lapin anti-BDNF

• Phare
• Validé par KD/KO

BDNF Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (2)

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(57)

Avis (1)

N° de cat : 25699-1-AP

Synonymes

Brain-derived neurotrophic factor, brain derived neurotrophic factor, BDNF precursor form

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Galerie de données de validation

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  WB analysis using 25699-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25699-1-AP (BDNF antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse cerebellum using 25699-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 25699-1-AP (BDNF antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse cerebellum using 25699-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 25699-1-AP (BDNF antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 25699-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25699-1-AP (BDNF antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 25699-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25699-1-AP (BDNF antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of PC-12 using 25699-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed PC-12 cells using BDNF antibody (25699-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, tissu cérébral de rat, tissu de cervelet de souris
• Résultats positifs en IHC: tissu de cervelet de souris, tissu cérébral de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules PC-12,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:400-1:1600
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 46 publications below
• IHC: See 11 publications below
• IF: See 7 publications below

Informations sur le produit

25699-1-AP cible BDNF dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, Lapin, souris, Macaque
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: BDNF Protéine recombinante Ag22489
• Nom complet: brain-derived neurotrophic factor
• Masse moléculaire calculée: 247 aa, 28 kDa
• Poids moléculaire observé: 30 kDa
• Numéro d’acquisition GenBank: BC029795
• Symbole du gène: BDNF
• Identification du gène (NCBI): 627
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

1) What is BDNF?

BDNF (brain-derived neurotrophic factor) is a small secreted growth factor that is important for the development and plasticity of the central nervous system and vital for long-term memory. Selected polymorphisms of BDNF have been shown to increase susceptibility to memory impairment and selected eating and mental disorders.

2) FAQs for BDNF

A) I can detect more than one band in my samples
BDNF is a neurotrophin that is a subject of maturation by proteolytic cleavage. The precursor protein (pre-proBDNF) is first cleaved to proBDNF (~34 kDa) by removing a signal peptide, and then to a mature form of BDNF (14 kDa). Additionally, (pre-)proBDNF can form dimers that run between 50 and 60 kDa, and a minor truncated form of proBDNF running at 28 kDa has also been reported (PMID: 11152678).

Publications

Species	Application	Title
mouse	WB	Mol Ther Nucleic Acids; Exploring the therapeutic potential of sγPNA-141: Pharmacodynamics and mechanistic insights during ischemic stroke recovery; Authors - Sanjeev Kumar Yadav
mouse	WB	Sci Signal; Glutamatergic argonaute2 promotes the formation of the neurovascular unit in mice; Authors - Chandan Sona
mouse	IHC	J Neurosci; Early BDNF Treatment Ameliorates Cell Loss in the Entorhinal Cortex of APP Transgenic Mice.; Authors - Nagahara Alan H AH
mouse	WB	Neuropsychopharmacology; CPEB3-dowregulated Nr3c1 mRNA translation confers resilience to developing posttraumatic stress disorder-like behavior in fear-conditioned mice.; Authors - Wen-Hsin Lu
mouse	WB	Cell Mol Life Sci; Furin promotes dendritic morphogenesis and learning and memory in transgenic mice.; Authors - Binglin Zhu
	IHC	J Neurotrauma; Bone Marrow Stromal Cell Intraspinal Transplants Fail to Improve Motor Outcomes in a Severe Model of Spinal Cord Injury.; Authors - John H Brock

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Armelle (Verified Customer) (09-03-2019)

This antibody worked very well. We were able to quantify expression levels in our area of interest.

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1/500
• Cell Tissue Type: bain slice fom the ventrolateral hypothalamus

BDNF Antibody Immunofluorescence, validation (1/500 dilution) in bain slice fom the ventrolateral hypothalamus (Cat no:25699-1-AP)

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