Anticorps Monoclonal anti-BDNF

BDNF Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1E5C5

N° de cat : 66292-1-PBS

Synonymes

Brain-derived neurotrophic factor, brain derived neurotrophic factor, BDNF precursor form, 1E5C5

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Galerie de données de validation

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  WB analysis of U-251 using 66292-1-Ig (same clone as 66292-1-PBS)

  U-251 cells were subjected to SDS PAGE followed by western blot with 66292-1-Ig (BDNF antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66292-1-PBS in a different storage buffer formulation.

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  WB analysis of fetal human brain using 66292-1-Ig (same clone as 66292-1-PBS)

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66292-1-Ig (BDNF Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66292-1-PBS in a different storage buffer formulation.

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  IF Staining of U-251 using 66292-1-Ig (same clone as 66292-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed U-251 cells using BDNF antibody (66292-1-Ig, Clone: 1E5C5 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66292-1-PBS in a different storage buffer formulation.

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  IF Staining of PC-12 using 66292-1-Ig (same clone as 66292-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed PC-12 cells using BDNF antibody (66292-1-Ig, Clone: 1E5C5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66292-1-PBS in a different storage buffer formulation.

• 

  IF Staining of PC-12 using 66292-1-Ig (same clone as 66292-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed PC-12 cells using BDNF antibody (66292-1-Ig, Clone: 1E5C5 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66292-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66292-1-PBS cible BDNF dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: BDNF Protéine recombinante Ag11329
• Nom complet: brain-derived neurotrophic factor
• Masse moléculaire calculée: 247 aa, 28 kDa
• Poids moléculaire observé: 32 kDa
• Numéro d’acquisition GenBank: BC029795
• Symbole du gène: BDNF
• Identification du gène (NCBI): 627
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

1) What is BDNF?

BDNF (brain-derived neurotrophic factor) is a small secreted growth factor that is important for the development and plasticity of the central nervous system and vital for long-term memory. Selected polymorphisms of BDNF have been shown to increase susceptibility to memory impairment and selected eating and mental disorders.

2) FAQs for BDNF

A) I can detect more than one band in my samples
BDNF is a neurotrophin that is a subject of maturation by proteolytic cleavage. The precursor protein (pre-proBDNF) is first cleaved to proBDNF (~34 kDa) by removing a signal peptide, and then to a mature form of BDNF (14 kDa). Additionally, (pre-)proBDNF can form dimers that run between 50 and 60 kDa, and a minor truncated form of proBDNF running at 28 kDa has also been reported (PMID: 11152678).

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