Anticorps Monoclonal anti-BDNF
BDNF Monoclonal Antibody for IF/ICC, FC (Intra)
Hôte / Isotype
Mouse / IgG2a
Réactivité testée
Humain
Applications
IF/ICC, FC (Intra)
Conjugaison
CoraLite®594 Fluorescent Dye
CloneNo.
1E5C5
N° de cat : CL594-66292
Synonymes
Galerie de données de validation
Applications testées
| Résultats positifs en IF/ICC | cellules PC-12, |
| Résultats positifs en FC (Intra) | cellules U-251, |
Dilution recommandée
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Informations sur le produit
CL594-66292 cible BDNF dans les applications de IF/ICC, FC (Intra) et montre une réactivité avec des échantillons Humain
| Réactivité | Humain |
| Hôte / Isotype | Mouse / IgG2a |
| Clonalité | Monoclonal |
| Type | Anticorps |
| Immunogène | BDNF Protéine recombinante Ag11329 |
| Nom complet | brain-derived neurotrophic factor |
| Masse moléculaire calculée | 247 aa, 28 kDa |
| Poids moléculaire observé | 32 kDa |
| Numéro d’acquisition GenBank | BC029795 |
| Symbole du gène | BDNF |
| Identification du gène (NCBI) | 627 |
| Conjugaison | CoraLite®594 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 588 nm / 604 nm |
| Forme | Liquide |
| Méthode de purification | Purification par protéine A |
| Tampon de stockage | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA |
| Conditions de stockage | Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA. |
Informations générales
1) What is BDNF?
BDNF (brain-derived neurotrophic factor) is a small secreted growth factor that is important for the development and plasticity of the central nervous system and vital for long-term memory. Selected polymorphisms of BDNF have been shown to increase susceptibility to memory impairment and selected eating and mental disorders.
2) FAQs for BDNF
A) I can detect more than one band in my samples
BDNF is a neurotrophin that is a subject of maturation by proteolytic cleavage. The precursor protein (pre-proBDNF) is first cleaved to proBDNF (~34 kDa) by removing a signal peptide, and then to a mature form of BDNF (14 kDa). Additionally, (pre-)proBDNF can form dimers that run between 50 and 60 kDa, and a minor truncated form of proBDNF running at 28 kDa has also been reported (PMID: 11152678).
Protocole
| Product Specific Protocols | |
|---|---|
| IF protocol for CL594 BDNF antibody CL594-66292 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |

