Anticorps Polyclonal de lapin anti-BEX1/2

BEX1/2 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(6)

Avis (1)

N° de cat : 12390-1-AP

Synonymes

BEX1, BEX1/2, BEX2, brain expressed X linked 2, hBex2, Protein BEX2

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Galerie de données de validation

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  WB analysis of human brain using 12390-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12390-1-AP (BEX1/2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human medulloblastoma using 12390-1-AP

  Immunohistochemical analysis of paraffin-embedded human medulloblastoma using 12390-1-AP (BEX1/2 antibody) at dilution of 1:100 (under 10x lens).

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Applications testées

• Résultats positifs en WB: tissu cérébral humain
• Résultats positifs en IHC: tissu de médulloblastome humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Informations sur le produit

12390-1-AP cible BEX1/2 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: BEX1/2 Protéine recombinante Ag3066
• Nom complet: brain expressed X-linked 2
• Masse moléculaire calculée: 128 aa, 15 kDa
• Poids moléculaire observé: 10-15 kDa
• Numéro d’acquisition GenBank: BC015522
• Symbole du gène: BEX2
• Identification du gène (NCBI): 84707
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

BEX1 is a signaling adapter molecule involved in p75NTR/NGFR signaling. It lays a role in cell cycle progression and neuronal differentiation. BEX1 inhibits neuronal differentiation in response to nerve growth factor (NGF). BEX2 is a regulator of mitochondrial apoptosis and G1 cell cycle in breast cancer. It regulates the level of PP2A regulatory subunit B and PP2A phosphatase activity. The homology between human BEX1 and BEX2 is 94%. This antibody detects both BEX1 and BEX2.

Publications

Species	Application	Title
human,mouse	IF	BMC Biol; Bex1 is essential for ciliogenesis and harbours biomolecular condensate-forming capacity.; Authors - Emi Hibino
human	WB	Int J Biol Sci; BEX2 promotes tumor proliferation in colorectal cancer.; Authors - Yeting Hu
human	WB	Front Cell Dev Biol; Single-cell and genetic multi-omics analysis combined with experiments confirmed the signature and potential targets of cuproptosis in hepatocellular carcinoma; Authors - Feng Cao
human,mouse	IHC,IF	Cell Death Dis; Crotonylated BEX2 interacts with NDP52 and enhances mitophagy to modulate chemotherapeutic agent-induced apoptosis in non-small-cell lung cancer cells; Authors - Ning Mu
human	WB	Aging (Albany NY); Machine learning-based identification and immune characterization of ferroptosis-related molecular clusters in osteoarthritis and validation; Authors - Xiaocheng Guo
human	WB	Transl Oncol; LMO2 confers value as a potential immunotherapy marker in pan-cancer analysis and inhibits progression of Clear Cell Renal Cell Carcinoma; Authors - Huiping Wang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Kamal (Verified Customer) (02-15-2024)

This antibody works for mouse liver tissue when diluted in 1X TBST.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mouse liver tissue

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