Anticorps Polyclonal de lapin anti-Osteocalcin/OCN

Osteocalcin/OCN Polyclonal Antibody for IHC, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

Publications(10)

N° de cat : 16157-1-AP

Synonymes

BGLAP, Osteocalcin, BGP, Bone Gla protein, Gamma-carboxyglutamic acid-containing protein

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Galerie de données de validation

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  IHC staining of human osteosarcoma using 16157-1-AP

  Immunohistochemical analysis of paraffin-embedded human osteosarcoma tissue slide using 16157-1-AP (Osteocalcin antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human osteosarcoma using 16157-1-AP

  Immunohistochemical analysis of paraffin-embedded human osteosarcoma tissue slide using 16157-1-AP (Osteocalcin antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat lung using 16157-1-AP

  Immunohistochemical analysis of paraffin-embedded rat lung tissue slide using 16157-1-AP (Osteocalcin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse lung using 16157-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 16157-1-AP (Osteocalcin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of NIH/3T3 using 16157-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using Osteocalcin/OCN antibody (16157-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  FC experiment of U2OS using 16157-1-AP

  1x10^6 U2OS cells were intracellularly stained with 0.4 ug Osteocalcin/OCN Polyclonal antibody (16157-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en IHC: tissu d'ostéosarcome humain, tissu pulmonaire de rat, tissu pulmonaire de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules NIH/3T3,
• Résultats positifs en FC (Intra): cellules U2OS,

Dilution recommandée

• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IHC: See 2 publications below
• IF: See 5 publications below

Informations sur le produit

16157-1-AP cible Osteocalcin/OCN dans les applications de WB, IHC, IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: bone gamma-carboxyglutamate (gla) protein
• Masse moléculaire calculée: 11 kDa
• Numéro d’acquisition GenBank: NM_199173
• Symbole du gène: Osteocalcin
• Identification du gène (NCBI): 632
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
rat	IF	ACS Appl Mater Interfaces; Cerium-Organic Framework and Resveratrol Composite Hydrogel Scaffold with Dual Antioxidant Activity for Enhanced Bone Regeneration; Authors - Yao Lu
mouse	IF	Aging (Albany NY); Primary cilia regulate gastric cancer-induced bone loss via cilia/Wnt/β-catenin signaling pathway.; Authors - Jie Xu
mouse	IHC	Front Pharmacol; Naringenin is a Potential Anabolic Treatment for Bone Loss by Modulating Osteogenesis, Osteoclastogenesis, and Macrophage Polarization.; Authors - Xin Zhou
mouse	IHC	Bone; Macrophage migration inhibitory factor promotes heterotopic ossification by mediating ROS/HIF-1α positive feedback loop and activating Wnt/β-catenin signaling pathway; Authors - Ping Li
mouse	WB	J Cell Mol Med; KIAA0753 enhances osteoblast differentiation suppressed by diabetes; Authors - Mengxue Li
rat	IF	Sci Rep; Ginsenoside Rg1 regulating inflammatory response and bone-remodeling through Keap1/Nrf2 signaling pathway in rats with periodontitis; Authors - Yang Zhou

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