Anticorps Polyclonal de lapin anti-BHMT

BHMT Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (3)

Applications

WB, IP, IHC, ELISA

Conjugaison

Non conjugué

Publications

31

Avis (1)

N° de cat : 15965-1-AP

Synonymes

BHMT

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Galerie de données de validation

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  WB analysis of mouse kidney using 15965-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 15965-1-AP (BHMT antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 15965-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 15965-1-AP (BHMT antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human kidney using 15965-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 15965-1-AP (BHMT antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse kidney using 15965-1-AP

  IP Result of anti-BHMT (IP:15965-1-AP, 4ug; Detection:15965-1-AP 1:1000) with mouse kidney tissue lysate 4400ug.

• 

  IHC staining of human spleen using 15965-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 15965-1-AP (BHMT antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human spleen using 15965-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 15965-1-AP (BHMT antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human skin using 15965-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 15965-1-AP (BHMT antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human heart using 15965-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 15965-1-AP (BHMT antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human heart using 15965-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 15965-1-AP (BHMT antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human placenta using 15965-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 15965-1-AP (BHMT antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human placenta using 15965-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 15965-1-AP (BHMT antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human skin using 15965-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 15965-1-AP (BHMT antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human lung using 15965-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 15965-1-AP (BHMT antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human lung using 15965-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 15965-1-AP (BHMT antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human ovary using 15965-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 15965-1-AP (BHMT antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human ovary using 15965-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 15965-1-AP (BHMT antibody) at dilution of 1:100 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu rénal de souris, cellules L02, tissu rénal humain
• Résultats positifs en IP: tissu rénal de souris
• Résultats positifs en IHC: tissu splénique humain, tissu cardiaque humain, tissu cutané humain, tissu ovarien humain, tissu placentaire humain, tissu pulmonaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 31 publications below
• IHC: See 1 publications below

Informations sur le produit

15965-1-AP cible BHMT dans les applications de WB, IP, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, porc, poulet, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: BHMT Protéine recombinante Ag8738
• Nom complet: betaine-homocysteine methyltransferase
• Masse moléculaire calculée: 406 aa, 45 kDa
• Poids moléculaire observé: 45-50 kDa
• Numéro d’acquisition GenBank: BC012616
• Symbole du gène: BHMT
• Identification du gène (NCBI): 635
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	Mol Cell; mTORC1 stimulates cell growth through SAM synthesis and m6A mRNA-dependent control of protein synthesis.; Authors - Elodie Villa
mouse	WB	NPJ Sci Food; Ablation of the gut microbiota alleviates high-methionine diet-induced hyperhomocysteinemia and glucose intolerance in mice; Authors - Wenqiang Li
	WB	J Anim Sci Biotechnol; Dietary betaine activates hepaticVTGIIexpression in laying hens associated with hypomethylation ofGRgene promoter and enhancedGRexpression.; Authors - Nagmeldin A Omer
rat	WB	J Agric Food Chem; Mitotically stable modification of DNA methylation in IGF2/H19 imprinting control region is associated with activated hepatic IGF2 expression in offspring rats from betaine-supplemented dams.; Authors - Shu Yang
pig	WB	J Agric Food Chem; Maternal Betaine Supplementation during Gestation Enhances Expression of mtDNA-Encoded Genes through D-Loop DNA Hypomethylation in the Skeletal Muscle of Newborn Piglets.; Authors - Yimin Jia
pig	WB	Sci Rep; Abnormally activated one-carbon metabolic pathway is associated with mtDNA hypermethylation and mitochondrial malfunction in the oocytes of polycystic gilt ovaries.; Authors - Longfei Jia

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Fatemeh (Verified Customer) (05-22-2023)

50 ug of protein was used for WB. Blot was blocked with milk and incubated with 1-1000 primary antibody overnight at 4C.

• Applications: Western Blot
• Primary Antibody Dilution: 1-1000
• Cell Tissue Type: ESC

BHMT Antibody Western Blot validation (1-1000 dilution) in ESC (Cat no:15965-1-AP)