Anticorps Recombinant de lapin anti-BHMT

BHMT Recombinant Antibody for WB, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat

Applications

WB, IP, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

250978E3

N° de cat : 86372-1-PBS

Synonymes

Betaine--homocysteine S-methyltransferase 1, EC:2.1.1.5

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Galerie de données de validation

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  WB analysis using 86372-1-RR (same clone as 86372-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86372-1-RR (BHMT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86372-1-PBS in a different storage buffer formulation.

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  IP experiment of rat liver using 86372-1-RR (same clone as 86372-1-PBS)

  IP result of anti-BHMT (IP:86372-1-RR, 4ug; Detection:86372-1-RR 1:3000) with rat liver tissue lysate 3080 ug. This data was developed using the same antibody clone with 86372-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 86372-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86372-1-RR against Human BHMT were performed. The affinity constant is below 1 pM.

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Informations sur le produit

86372-1-PBS cible BHMT dans les applications de WB, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: BHMT Protéine recombinante Ag8738
• Nom complet: betaine-homocysteine methyltransferase
• Masse moléculaire calculée: 406 aa, 45 kDa
• Poids moléculaire observé: 45 kDa
• Numéro d’acquisition GenBank: BC012616
• Symbole du gène: BHMT
• Identification du gène (NCBI): 635
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Betaine-homocysteine methyltransferase (BHMT) is a cytosolic enzyme that Involved in the regulation of homocysteine metabolism. BHMT catalyzes the conversion of betaine and homocysteine to dimethylglycine and methionine, respectively. This reaction is also required for the irreversible oxidation of choline(PMID: 8798461, 10529246).

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