Anticorps Polyclonal de lapin anti-BID

• Phare
• Validé par KD/KO

BID Polyclonal Antibody for IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (3)

Applications

WB, IP, IHC, ELISA

Conjugaison

Non conjugué

Publications

51

N° de cat : 10988-1-AP

Synonymes

BID, FP497, p11 BID, p22 BID

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Galerie de données de validation

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  WB analysis using 10988-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10988-1-AP (BID antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10988-1-AP

  WB result of BID antibody (10988-1-AP, 1:8000) with si-Control and si-BID transfected HeLa cells.

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  WB analysis of HeLa using 10988-1-AP

  Tunicamycin treated HeLa cells were subjected to SDS PAGE followed by western blot with 10988-1-AP (BID Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 10988-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 10988-1-AP (BID antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 10988-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 10988-1-AP (BID antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 10988-1-AP

  IP result of anti-BID (IP:10988-1-AP, 4ug; Detection:10988-1-AP 1:500) with HeLa cells lysate 1280 ug.

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Applications testées

• Résultats positifs en WB: cellules A431, cellules A549, cellules HEK-293, cellules HeLa traitées à la tunicamycine, cellules HepG2, cellules Jurkat, tissu cérébral humain
• Résultats positifs en IP: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 50 publications below
• IHC: See 1 publications below

Informations sur le produit

10988-1-AP cible BID dans les applications de WB, IP, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: BID Protéine recombinante Ag1445
• Nom complet: BH3 interacting domain death agonist
• Masse moléculaire calculée: 22 kDa
• Poids moléculaire observé: 22 kDa
• Numéro d’acquisition GenBank: BC009197
• Symbole du gène: BID
• Identification du gène (NCBI): 637
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

BID, also named as p22 BID, can be cleaved into p11 BID, p13 BID and p15 BID. It is pro-apoptotic molecules. The major proteolytic product p15 BID allows the release of cytochrome c. BID-L, BID-EL and BID-ES induce ICE-like proteases and apoptosis. BID-S does not induce apoptosis. BID counters the protective effect of Bcl-2. (PMID:14583606)

Publications

Species	Application	Title
rat	WB	Bioact Mater; A ROS-responsive, self-immolative and self-reporting hydrogen sulfide donor with multiple biological activities for the treatment of myocardial infarction.; Authors - Mengyun Yao
mouse	WB	Environ Pollut; Silica nanoparticles inducing the apoptosis via microRNA-450b-3p targeting MTCH2 in mice and spermatocyte cell.; Authors - Guiqing Zhou
rat	WB	Cell Mol Life Sci; Clostridioides difficile toxin B alone and with pro-inflammatory cytokines induces apoptosis in enteric glial cells by activating three different signalling pathways mediated by caspases, calpains and cathepsin B.; Authors - Katia Fettucciari
human	WB	Int J Cancer; Tetrandrine induces apoptosis by activating reactive oxygen species and repressing Akt activity in human hepatocellular carcinoma.; Authors - Liu Chaoyang C
human	WB	Free Radic Biol Med; Shikonin, a Chinese plant-derived naphthoquinone, induces apoptosis in hepatocellular carcinoma cells through reactive oxygen species: A potential new treatment for hepatocellular carcinoma.; Authors - Gong Ke K
rat	WB	Stem Cell Res Ther; Adipose-derived stem cells alleviate radiation-induced dermatitis by suppressing apoptosis and downregulating cathepsin F expression.; Authors - Chaoling Yao

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