Anticorps Polyclonal de lapin anti-BIN1

• Phare
• Validé par KD/KO

BIN1 Polyclonal Antibody for WB, IHC, IF-P, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF-P, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 14647-1-PBS

Synonymes

---

Galerie de données de validation

• 

  WB analysis using 14647-1-AP (same clone as 14647-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 14647-1-AP (BIN1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14647-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse brain using 14647-1-AP (same clone as 14647-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14647-1-AP (BIN1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14647-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 14647-1-AP (same clone as 14647-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14647-1-AP (BIN1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14647-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse skeletal muscle using 14647-1-AP (same clone as 14647-1-PBS)

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 14647-1-AP (BIN1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14647-1-PBS in a different storage buffer formulation.

• 

  IP experiment of mouse brain using 14647-1-AP (same clone as 14647-1-PBS)

  IP result of anti-BIN1 (IP:14647-1-AP, 4ug; Detection:14647-1-AP 1:500) with mouse brain tissue lysate 3440ug. This data was developed using the same antibody clone with 14647-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse skeletal muscle using 14647-1-AP (same clone as 14647-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 14647-1-AP (BIN1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14647-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse skeletal muscle using 14647-1-AP (same clone as 14647-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 14647-1-AP (BIN1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14647-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human osteosarcoma using 14647-1-AP (same clone as 14647-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human osteosarcoma using 14647-1-AP (BIN1 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 14647-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human osteosarcoma using 14647-1-AP (same clone as 14647-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human osteosarcoma using 14647-1-AP (BIN1 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 14647-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 14647-1-AP (same clone as 14647-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14647-1-AP (BIN1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14647-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 14647-1-AP (same clone as 14647-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14647-1-AP (BIN1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14647-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse brain using 14647-1-AP (same clone as 14647-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using BIN1 antibody (14647-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14647-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse brain using 14647-1-AP (same clone as 14647-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using BIN1 antibody (14647-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14647-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

14647-1-PBS cible BIN1 dans les applications de WB, IHC, IF-P, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: BIN1 Protéine recombinante Ag6240
• Nom complet: bridging integrator 1
• Masse moléculaire calculée: 65 kDa
• Poids moléculaire observé: 50-65 kDa
• Numéro d’acquisition GenBank: BC004101
• Symbole du gène: BIN1
• Identification du gène (NCBI): 274
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

BIN1 (Bridging integrator 1), also known as amphiphysin II or Myc box-dependent-interacting protein 1, is a ubiquitous nucleocytoplasmic adaptor protein that was identified initially as an MYC-interacting proapoptotic tumor suppressor. Alternative splicing of the gene results in multiple transcript variants encoding different isoforms. BIN1 is a key regulator of different cellular functions, including endocytosis and membrane recycling, cytoskeleton regulation, DNA repair, cell cycle progression, and apoptosis (PMID: 24590001).

{{ptg:RelatedPrimaryAntibodies}}