Anticorps Polyclonal de lapin anti-BIN2

• Phare
• Validé par KD/KO

BIN2 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 14245-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 14245-1-AP (same clone as 14245-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 14245-1-AP (BIN2 antibody) at dilution of 1:7000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14245-1-PBS in a different storage buffer formulation.

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  WB analysis of U-937 using 14245-1-AP (same clone as 14245-1-PBS)

  U-937 cells were subjected to SDS PAGE followed by western blot with 14245-1-AP (BIN2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14245-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 14245-1-AP (same clone as 14245-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14245-1-AP (BIN2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14245-1-PBS in a different storage buffer formulation.

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  IP experiment of Jurkat using 14245-1-AP (same clone as 14245-1-PBS)

  IP result of anti-BIN2 (IP:14245-1-AP, 4ug; Detection:14245-1-AP 1:400) with Jurkat cells lysate 2000ug. This data was developed using the same antibody clone with 14245-1-PBS in a different storage buffer formulation.

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  IHC staining of human spleen using 14245-1-AP (same clone as 14245-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human spleen using 14245-1-AP (BIN2 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 14245-1-PBS in a different storage buffer formulation.

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  IHC staining of human spleen using 14245-1-AP (same clone as 14245-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human spleen using 14245-1-AP (BIN2 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 14245-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung using 14245-1-AP (same clone as 14245-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung using 14245-1-AP (BIN2 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 14245-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung using 14245-1-AP (same clone as 14245-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung using 14245-1-AP (BIN2 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 14245-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary using 14245-1-AP (same clone as 14245-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary using 14245-1-AP (BIN2 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 14245-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary using 14245-1-AP (same clone as 14245-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary using 14245-1-AP (BIN2 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 14245-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 14245-1-AP (same clone as 14245-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 14245-1-AP (BIN2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14245-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 14245-1-AP (same clone as 14245-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 14245-1-AP (BIN2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14245-1-PBS in a different storage buffer formulation.

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  IHC staining of human heart using 14245-1-AP (same clone as 14245-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart using 14245-1-AP (BIN2 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 14245-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 14245-1-AP (same clone as 14245-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart using 14245-1-AP (BIN2 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 14245-1-PBS in a different storage buffer formulation.

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  IHC staining of human skin using 14245-1-AP (same clone as 14245-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin using 14245-1-AP (BIN2 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 14245-1-PBS in a different storage buffer formulation.

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  IHC staining of human skin using 14245-1-AP (same clone as 14245-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin using 14245-1-AP (BIN2 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 14245-1-PBS in a different storage buffer formulation.

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Informations sur le produit

14245-1-PBS cible BIN2 dans les applications de WB, IHC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: BIN2 Protéine recombinante Ag5502
• Nom complet: bridging integrator 2
• Masse moléculaire calculée: 62 kDa
• Poids moléculaire observé: 80-90 kDa
• Numéro d’acquisition GenBank: BC047686
• Symbole du gène: BIN2
• Identification du gène (NCBI): 51411
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

BAR proteins, which are characterized by a common N-terminal BAR domain, are a family of adaptor proteins implicated in a diverse set of cellular processes. BIN1 (bridging integrator 1) and BIN2 are BAR proteins. The human BIN2 gene is located at chromosome 4q22.1, and encodes a 565-amino acid protein with a predicted molecular weight of ~62 kDa. It shows preferential expression in hematopoietic tissues, with highest mRNA levels in spleen and peripheral blood leukocytes and also high levels in thymus, colon, and placenta. BIN2 has been shown to migrate with an apparent mobility of ~80 kDa, and interact with BIN1. (PMID: 10903846)

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