Anticorps Polyclonal de lapin anti-BIN3

BIN3 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 11215-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of K-562 using 11215-1-AP (same clone as 11215-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 11215-1-AP (BIN3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11215-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 11215-1-AP (same clone as 11215-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 11215-1-AP (BIN3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11215-1-PBS in a different storage buffer formulation.

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  IP experiment of K-562 using 11215-1-AP (same clone as 11215-1-PBS)

  IP result of anti-BIN3 (IP:11215-1-AP, 4ug; Detection:11215-1-AP 1:300) with K-562 cells lysate 3200ug. This data was developed using the same antibody clone with 11215-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 11215-1-AP (same clone as 11215-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11215-1-AP (BIN3 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11215-1-PBS in a different storage buffer formulation.

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Informations sur le produit

11215-1-PBS cible BIN3 dans les applications de WB, IHC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: BIN3 Protéine recombinante Ag1713
• Nom complet: bridging integrator 3
• Masse moléculaire calculée: 30 kDa
• Poids moléculaire observé: 30 kDa
• Numéro d’acquisition GenBank: BC009824
• Symbole du gène: BIN3
• Identification du gène (NCBI): 55909
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Bridging integrator 3 (BIN3) is a conserved BAR (Bin/amphiphysin/ Rvs) domain adaptor protein (PMID: 11274158). BAR domain proteins, sharing an N-terminal BAR domain, have been implicated in diverse cellular processes, including endocytosis, vesicle fusion, cell polarity, actin organization, signal transduction, transcription repression, and tumor suppression (PMID: 16524918). BIN3 is involved in cytokinesis and septation where it has a role in the localization of F-actin (PMID: 11274158).

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