Anticorps Recombinant de lapin anti-BLIMP1/PRDM1

BLIMP1/PRDM1 Recombinant Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

242030B1

N° de cat : 84625-3-RR

Synonymes

BLIMP1, PRDM1, 242030B1, BLIMP 1, PRDI BF1

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Galerie de données de validation

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  WB analysis using 84625-3-RR

  HeLa cells were subjected to SDS PAGE followed by western blot with 84625-3-RR (BLIMP1/PRDM1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of A431 using 84625-3-RR

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using BLIMP1/PRDM1 antibody (84625-3-RR, Clone: 242030B1 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  IF Staining of A549 using 84625-3-RR

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using BLIMP1/PRDM1 antibody (84625-3-RR, Clone: 242030B1 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules A431
• Résultats positifs en IF/ICC: cellules A549, cellules A431

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84625-3-RR cible BLIMP1/PRDM1 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: BLIMP1/PRDM1 Protéine recombinante Eg2625
• Nom complet: PR domain containing 1, with ZNF domain
• Masse moléculaire calculée: 92 kDa
• Poids moléculaire observé: 96-110 kDa
• Numéro d’acquisition GenBank: NM_001198.3
• Symbole du gène: PRDM1
• Identification du gène (NCBI): 639
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

BLIMP1(B lymphocyte-induced maturation protein 1), also known as PRDM1(PR domain zinc finger protein 1), is an evolutionarily conserved transcriptional regulator originally described as a repressor of gene transcription(PMID: 35154079). BLIMP1/PRDM1 is widely expressed in multiple cell types including B lymphocytes, T lymphocytes, mammary luminal progenitor cells and skin epithelial cells. BLIMP1/PRDM1 is a master regulator of terminal B cell differentiation and may also function as a tumor suppressor in the pathogenesis of DLBCL, where it is frequently inactivated by mutations and deletions.

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