Anticorps Polyclonal de lapin anti-BLK

BLK Polyclonal Antibody for WB, IHC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IP, ELISA

Conjugaison

Non conjugué

Publications(3)

N° de cat : 10510-1-AP

Synonymes

B lymphocyte kinase, B lymphoid tyrosine kinase, EC:2.7.10.2, p55 Blk, p55-Blk

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Galerie de données de validation

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  WB analysis of Jurkat using 10510-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10510-1-AP (BLK antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 10510-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 10510-1-AP (BLK antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 10510-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 10510-1-AP (BLK antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 10510-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 10510-1-AP (BLK Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of SH-SY5Y using 10510-1-AP

  IP result of anti-BLK (IP:10510-1-AP, 4ug; Detection:10510-1-AP 1:500) with SH-SY5Y cells lysate 1600ug.

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  IHC staining of human pancreas cancer using 10510-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10510-1-AP (BLK Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 10510-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10510-1-AP (BLK Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules Raji, cellules SH-SY5Y
• Résultats positifs en IP: cellules SH-SY5Y
• Résultats positifs en IHC: tissu de cancer du pancréas humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IP: See 1 publications below

Informations sur le produit

10510-1-AP cible BLK dans les applications de WB, IHC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: BLK Protéine recombinante Ag0795
• Nom complet: B lymphoid tyrosine kinase
• Masse moléculaire calculée: 58 kDa
• Poids moléculaire observé: 58 kDa
• Numéro d’acquisition GenBank: BC007371
• Symbole du gène: BLK
• Identification du gène (NCBI): 640
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Tyrosine-protein kinase Blk(BLK) is typically involved in cell proliferation and differentiation.BLK is a 58 kDa protein with important role in B-cell receptor signaling and B-cell development. BLK also stimulates INS synthesis and secretion in response to glucose and enhances the expression of several pancreatic beta-cell transcription factors.

Publications

Species	Application	Title
human	WB,IP	Signal Transduct Target Ther; B-lymphoid tyrosine kinase-mediated FAM83A phosphorylation elevates pancreatic tumorigenesis through interacting with β-catenin; Authors - Cefan Zhou
human	WB	Cancer Cell; Molecular targets of glucocorticoids that elucidate their therapeutic efficacy in aggressive lymphomas; Authors - Jaewoo Choi
		Diabet Med; A novel BLK heterozygous mutation (p.Met121lle) in maturity-onset diabetes mellitus: A case report and literature review; Authors - Fenjuan Xu

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