Anticorps Polyclonal de lapin anti-BLK

BLK Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 10510-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of Jurkat using 10510-1-AP (same clone as 10510-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10510-1-AP (BLK antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10510-1-PBS in a different storage buffer formulation.

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  WB analysis of Raji using 10510-1-AP (same clone as 10510-1-PBS)

  Raji cells were subjected to SDS PAGE followed by western blot with 10510-1-AP (BLK antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10510-1-PBS in a different storage buffer formulation.

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  WB analysis of SH-SY5Y using 10510-1-AP (same clone as 10510-1-PBS)

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 10510-1-AP (BLK Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10510-1-PBS in a different storage buffer formulation.

• 

  WB analysis of SH-SY5Y using 10510-1-AP (same clone as 10510-1-PBS)

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 10510-1-AP (BLK antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10510-1-PBS in a different storage buffer formulation.

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  IP experiment of SH-SY5Y using 10510-1-AP (same clone as 10510-1-PBS)

  IP result of anti-BLK (IP:10510-1-AP, 4ug; Detection:10510-1-AP 1:500) with SH-SY5Y cells lysate 1600ug. This data was developed using the same antibody clone with 10510-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 10510-1-AP (same clone as 10510-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10510-1-AP (BLK Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10510-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 10510-1-AP (same clone as 10510-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10510-1-AP (BLK Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10510-1-PBS in a different storage buffer formulation.

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Informations sur le produit

10510-1-PBS cible BLK dans les applications de WB, IHC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: BLK Protéine recombinante Ag0795
• Nom complet: B lymphoid tyrosine kinase
• Masse moléculaire calculée: 58 kDa
• Poids moléculaire observé: 58 kDa
• Numéro d’acquisition GenBank: BC007371
• Symbole du gène: BLK
• Identification du gène (NCBI): 640
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Tyrosine-protein kinase Blk(BLK) is typically involved in cell proliferation and differentiation.BLK is a 58 kDa protein with important role in B-cell receptor signaling and B-cell development. BLK also stimulates INS synthesis and secretion in response to glucose and enhances the expression of several pancreatic beta-cell transcription factors.

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