Anticorps Monoclonal anti-BLNK

BLNK Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

1A1A1

N° de cat : 66953-1-Ig

Synonymes

B cell linker, B cell linker protein, BASH, BLNK, BLNK S, Cytoplasmic adapter protein, LY57, SLP 65, SLP65

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Galerie de données de validation

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  WB analysis of human spleen using 66953-1-Ig

  human spleen tissue were subjected to SDS PAGE followed by western blot with 66953-1-Ig (BLNK antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Ramos using 66953-1-Ig

  Ramos cells were subjected to SDS PAGE followed by western blot with 66953-1-Ig (BLNK antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig spleen using 66953-1-Ig

  pig spleen tissue were subjected to SDS PAGE followed by western blot with 66953-1-Ig (BLNK antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 66953-1-Ig

  Raji cells were subjected to SDS PAGE followed by western blot with 66953-1-Ig (BLNK antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Daudi using 66953-1-Ig

  Daudi cells were subjected to SDS PAGE followed by western blot with 66953-1-Ig (BLNK antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human appendicitis using 66953-1-Ig

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66953-1-Ig (BLNK antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human appendicitis using 66953-1-Ig

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66953-1-Ig (BLNK antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse thymus using 66953-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse thymus tissue slide using 66953-1-Ig (BLNK antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse thymus using 66953-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse thymus tissue slide using 66953-1-Ig (BLNK antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat spleen using 66953-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 66953-1-Ig (BLNK antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat spleen using 66953-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 66953-1-Ig (BLNK antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of Raji using 66953-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed Raji cells using BLNK antibody (66953-1-Ig, Clone: 1A1A1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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  IF Staining of NIH/3T3 using 66953-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using BLNK antibody (66953-1-Ig, Clone: 1A1A1 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

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Applications testées

• Résultats positifs en WB: tissu splénique humain, cellules Daudi, cellules Raji, cellules Ramos, tissu splénique de porc
• Résultats positifs en IHC: tissu d'appendicite humain, tissu de thymus de souris, tissu splénique de rat; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules Raji, cellules NIH/3T3

Dilution recommandée

• Western Blot (WB): WB : 1:3000-1:8000
• Immunohistochimie (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

66953-1-Ig cible BLNK dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: BLNK Protéine recombinante Ag28673
• Nom complet: B-cell linker
• Masse moléculaire calculée: 50 kDa
• Poids moléculaire observé: 65-70 kDa
• Numéro d’acquisition GenBank: BC018906
• Symbole du gène: BLNK
• Identification du gène (NCBI): 29760
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

BLNK (also known as SLP-65 or BASH) is an important adaptor protein selectively expressed in B-lineage cells. BLNK bridges B cell receptor-associated kinase activation with downstream signaling pathways, thereby affecting various biological functions. BLNK has been shown to be necessary for BCR-mediated Ca2+ mobilization, for the activation of mitogen-activated protein kinases such as ERK, JNK, and p38 in a chicken B cell line DT40, and for activation of transcription factors such as NF-AT and NF-κB in human or mouse B cells. BLNK plays a crucial role in pre-BCR-dependent progression of B cell development, BCR-mediated B cell survival, activation, proliferation, and T-independent immune responses.

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