Anticorps Monoclonal anti-BLNK

BLNK Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1A1A1

N° de cat : 66953-1-PBS

Synonymes

B cell linker, B cell linker protein, BASH, BLNK, BLNK S, Cytoplasmic adapter protein, LY57, SLP 65, SLP65

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Galerie de données de validation

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  WB analysis of human spleen using 66953-1-Ig (same clone as 66953-1-PBS)

  human spleen tissue were subjected to SDS PAGE followed by western blot with 66953-1-Ig (BLNK antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66953-1-PBS in a different storage buffer formulation.

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  WB analysis of Ramos using 66953-1-Ig (same clone as 66953-1-PBS)

  Ramos cells were subjected to SDS PAGE followed by western blot with 66953-1-Ig (BLNK antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66953-1-PBS in a different storage buffer formulation.

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  WB analysis of pig spleen using 66953-1-Ig (same clone as 66953-1-PBS)

  pig spleen tissue were subjected to SDS PAGE followed by western blot with 66953-1-Ig (BLNK antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66953-1-PBS in a different storage buffer formulation.

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  WB analysis of Raji using 66953-1-Ig (same clone as 66953-1-PBS)

  Raji cells were subjected to SDS PAGE followed by western blot with 66953-1-Ig (BLNK antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66953-1-PBS in a different storage buffer formulation.

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  WB analysis of Daudi using 66953-1-Ig (same clone as 66953-1-PBS)

  Daudi cells were subjected to SDS PAGE followed by western blot with 66953-1-Ig (BLNK antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66953-1-PBS in a different storage buffer formulation.

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  IHC staining of human appendicitis using 66953-1-Ig (same clone as 66953-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66953-1-Ig (BLNK antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66953-1-PBS in a different storage buffer formulation.

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  IHC staining of human appendicitis using 66953-1-Ig (same clone as 66953-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66953-1-Ig (BLNK antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66953-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse thymus using 66953-1-Ig (same clone as 66953-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse thymus tissue slide using 66953-1-Ig (BLNK antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66953-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse thymus using 66953-1-Ig (same clone as 66953-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse thymus tissue slide using 66953-1-Ig (BLNK antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66953-1-PBS in a different storage buffer formulation.

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  IHC staining of rat spleen using 66953-1-Ig (same clone as 66953-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 66953-1-Ig (BLNK antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66953-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat spleen using 66953-1-Ig (same clone as 66953-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 66953-1-Ig (BLNK antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66953-1-PBS in a different storage buffer formulation.

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  IF Staining of Raji using 66953-1-Ig (same clone as 66953-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Raji cells using BLNK antibody (66953-1-Ig, Clone: 1A1A1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66953-1-PBS in a different storage buffer formulation.

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  IF Staining of NIH/3T3 using 66953-1-Ig (same clone as 66953-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using BLNK antibody (66953-1-Ig, Clone: 1A1A1 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66953-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66953-1-PBS cible BLNK dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: BLNK Protéine recombinante Ag28673
• Nom complet: B-cell linker
• Masse moléculaire calculée: 50 kDa
• Poids moléculaire observé: 65-70 kDa
• Numéro d’acquisition GenBank: BC018906
• Symbole du gène: BLNK
• Identification du gène (NCBI): 29760
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

BLNK (also known as SLP-65 or BASH) is an important adaptor protein selectively expressed in B-lineage cells. BLNK bridges B cell receptor-associated kinase activation with downstream signaling pathways, thereby affecting various biological functions. BLNK has been shown to be necessary for BCR-mediated Ca2+ mobilization, for the activation of mitogen-activated protein kinases such as ERK, JNK, and p38 in a chicken B cell line DT40, and for activation of transcription factors such as NF-AT and NF-κB in human or mouse B cells. BLNK plays a crucial role in pre-BCR-dependent progression of B cell development, BCR-mediated B cell survival, activation, proliferation, and T-independent immune responses.

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