Anticorps Monoclonal anti-BMI1

• Phare
• Validé par KD/KO

BMI1 Monoclonal Antibody for WB, IHC, IF/ICC, IP, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, singe, souris

Applications

WB, IHC, IF/ICC, IP, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2E10A11

N° de cat : 66161-1-PBS

Synonymes

2E10A11, PCGF4, Polycomb complex protein BMI-1, Polycomb group RING finger protein 4, RNF51

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Galerie de données de validation

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  WB analysis of PC-12 using 66161-1-Ig (same clone as 66161-1-PBS)

  PC-12 cells were subjected to SDS PAGE followed by western blot with 66161-1-Ig (BMI1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66161-1-PBS in a different storage buffer formulation.

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  WB analysis of HT-1080 using 66161-1-Ig (same clone as 66161-1-PBS)

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 66161-1-Ig (BMI1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66161-1-PBS in a different storage buffer formulation.

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  WB analysis of U2OS using 66161-1-Ig (same clone as 66161-1-PBS)

  U2OS cells were subjected to SDS PAGE followed by western blot with 66161-1-Ig (BMI1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66161-1-PBS in a different storage buffer formulation.

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  WB analysis of U-937 using 66161-1-Ig (same clone as 66161-1-PBS)

  U-937 cells were subjected to SDS PAGE followed by western blot with 66161-1-Ig (BMI1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66161-1-PBS in a different storage buffer formulation.

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  IP experiment of HEK-293 using 66161-1-Ig (same clone as 66161-1-PBS)

  IP result of anti-BMI1 (IP:66161-1-Ig, 3ug; Detection:66161-1-Ig 1:1000) with HEK-293 cells lysate 1800ug. This data was developed using the same antibody clone with 66161-1-PBS in a different storage buffer formulation.

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  IHC staining of human lymphoma using 66161-1-Ig (same clone as 66161-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66161-1-Ig (BMI1 Antibody) at dilution of 1:50 under 40x lens. This data was developed using the same antibody clone with 66161-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66161-1-Ig (same clone as 66161-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66161-1-Ig (BMI1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66161-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 66161-1-Ig (same clone as 66161-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 66161-1-Ig (BMI1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66161-1-PBS in a different storage buffer formulation.

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  IHC staining of human lymphoma using 66161-1-Ig (same clone as 66161-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66161-1-Ig (BMI1 Antibody) at dilution of 1:50 under 10x lens. This data was developed using the same antibody clone with 66161-1-PBS in a different storage buffer formulation.

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  IF Staining of COS-7 using 66161-1-Ig (same clone as 66161-1-PBS)

  Immunofluorescent analysis of COS-7 cells using 66161-1-Ig (BMI1 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Mouse IgG. This data was developed using the same antibody clone with 66161-1-PBS in a different storage buffer formulation.

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  IF Staining of HEK-293 using 66161-1-Ig (same clone as 66161-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using BMI1 antibody (66161-1-Ig, Clone: 2E10A11 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66161-1-PBS in a different storage buffer formulation.

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  Cytometric bead array sample test of MP51443-1

  Cytometric bead array sample test of MP51443-1, BMI1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66161-2-PBS. Detection antibody: 66161-1-PBS.

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  Cytometric bead array standard curve of MP51443-1

  Cytometric bead array standard curve of MP51443-1, BMI1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66161-2-PBS. Detection antibody: 66161-1-PBS. Standard:Ag21284. Range: 0.391-100 ng/mL.

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Informations sur le produit

66161-1-PBS cible BMI1 dans les applications de WB, IHC, IF/ICC, IP, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain, singe, souris

• Réactivité: Humain, singe, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: BMI1 Protéine recombinante Ag21284
• Nom complet: BMI1 polycomb ring finger oncogene
• Masse moléculaire calculée: 37 kDa
• Poids moléculaire observé: 37-43 kDa
• Numéro d’acquisition GenBank: BC011652
• Symbole du gène: BMI1
• Identification du gène (NCBI): 648
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

BMI- 1 is one of polycomb group genes, which together with Ring1 strongly enhances the E3 ubiquitin ligase activity of the Ring2 catalytic subunit. Bmi1 plays an important role in the regulation of cell proliferation and senescence through repression of the p16Ink4a and p19Arf genes and is required for maintenance of adult hematopoietic and neural stem cells. The antibody reacts with the 37kd BMI1 protein.

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